WISP3 active protein

WISP3 protein

Cat. Num. AAA539485

• Gene Names: WISP3; PPD; CCN6; LIBC; PPAC; WISP-3
• Applications: User optimized
• Purity: > 98% pure
• Synonyms: WISP3; WISP3 protein; CCN6 protein; WISP 3 protein; WISP-3; WISP-3 protein; WNT-1 inducible signaling pathway protein-3 protein; WISP 3; WISP3 active protein

• Host: Human
• Purity/Purification: > 98% pure
• Form/Format: Supplied lyophilized from 10mM CH3COOH. Reconstitute in 10mM Acetic Acid o a concentration of 0.1 - 1.0 mg/ml.
• Sequence: Region of WISP3 protein corresponding to amino acids TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L.
• Sequence Length: 372

• Applicable Applications for WISP3 active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis and fetal kidney and weaker expression in placenta, ovary, prostate, small intestine, and skeletally-derived cells. WISP-3 is required for normal postnatal skeletal growth and cartilage homeostasis. Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot-like (CTCK) domain.
• Bioactivity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 ug/ml.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for WISP3 active protein

Purified recombinant Human WISP3 protein

Product Categories/Family for WISP3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 38202241
• NCBI GeneID: 8838
• NCBI Accession #: NP_937882.1
• NCBI GenBank Nucleotide #: NM_198239.1
• UniProt Accession #: O95389; Q3KR29; Q5H8W4; Q6UXH6
• Molecular Weight: 41,402 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 3 isoform 3
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 3
• NCBI Official Symbol: WISP3
• NCBI Official Synonym Symbols: PPD; CCN6; LIBC; PPAC; WISP-3
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 3
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 3
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP3
• UniProt Synonym Gene Names: CCN6; WISP-3
• UniProt Entry Name: WISP3_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WISP3: Appears to be required for normal postnatal skeletal growth and cartilage homeostasis. Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC). PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: extracellular space; proteinaceous extracellular matrix

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion

Disease: Arthropathy, Progressive Pseudorheumatoid, Of Childhood