Tie2, Receptor Tyrosine Kinase, N-terminal GST tag Active Protein | TEK active protein

Tie2, Receptor Tyrosine Kinase, Recombinant, Human, N-terminal GST tag (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Cat. Num. AAA636855

• Gene Names: TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
• Reactivity: Human
• Purity: 50% by SDS-PAGE
• Synonyms: Tie2; Receptor Tyrosine Kinase; N-terminal GST tag; Recombinant; Human; N-terminal GST tag (Tunica Internal Endothelial Cell Kinase; Angiopoietin1 Receptor; p140 Tek; Tyrosine Protein Kinase Receptor); TEK active protein

• Reactivity: Human
• Purity/Purification: 50% by SDS-PAGE
• Form/Format: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3mM DTT, 50% glycerol.

• Application Notes: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 140 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30 degree C.
• Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na orthovanadate, 0.5mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.2ug recombinant Tie2.
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for TEK active protein

Human Tie2 GenBank Accession No. NM_000459), (a.a. 771-end) with N-terminal His tag,

MW 45kD, expressed in an Baculovirus infected Sf9 cell expression system.

Product Categories/Family for TEK active protein

Molecular Biology; MB-Receptor Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 112180440
• NCBI GeneID: 7010
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 45kD
• NCBI Official Full Name: TEK tyrosine kinase, endothelial
• NCBI Official Synonym Full Names: TEK tyrosine kinase, endothelial
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2
• UniProt Protein Name: Angiopoietin-1 receptor
• Protein Family: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN

NCBI Description

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; Protein kinase, TK; TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: focal adhesion; cell surface; microvillus; integral to plasma membrane; basolateral plasma membrane; perinuclear region of cytoplasm; apical plasma membrane; extracellular region; plasma membrane; basal plasma membrane; stress fiber; actin filament; intercellular junction; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; growth factor binding; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; heart development; cell-matrix adhesion; protein amino acid autophosphorylation; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; endothelial cell proliferation; negative regulation of inflammatory response; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; positive regulation of protein amino acid phosphorylation; sprouting angiogenesis; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal