Thrombopoietin Active Protein | THPO active protein

Thrombopoietin protein

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Applications: User optimized
• Purity: > 98% pure; Thrombopoietin protein was purified by a proprietary chromatographic technique.
• Synonyms: Thrombopoietin; Thrombopoietin protein; THPO protein; MGDF protein; c-MPL Ligand protein; Megakaryocyte colony-stimulating factor protein; TPO protein; THPO active protein

• Host: Human
• Purity/Purification: > 98% pure; Thrombopoietin protein was purified by a proprietary chromatographic technique.
• Form/Format: Lyophilized from 5 mM Tris, pH 8.0. Reconstitute in 5mM Tris to a concentration of 0.5-1.0 mg/ml
• Sequence Length: 353

• Applicable Applications for THPO active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets
• Bioactivity: The ED50 as determined by the dose-dependant stimulation of MO7e cells is
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for THPO active protein

Purified recombinant Human Thrombopoietin protein

Product Categories/Family for THPO active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4507493
• NCBI GeneID: 7066
• NCBI Accession #: NP_000451.1
• NCBI GenBank Nucleotide #: NM_000460.2
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
• Molecular Weight: 18,608 Da
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

NCBI Description

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; platelet activation; multicellular organismal development; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1