TGF beta 1 active protein

TGF beta 1 protein

Cat. Num. AAA539454

• Applications: User optimized
• Purity: > 98% pure
• Synonyms: TGF beta 1; TGF beta 1 protein; TGF beta-1 protein; TGFB1 protein; TGF-1b protein; TGF beta-1; Differentiation inhibiting factor protein; Cartilage-inducing factor protein; Transforming Growth Factor-beta1 protein; TGF beta 1 active protein

• Host: Human
• Purity/Purification: > 98% pure
• Form/Format: Lyophilized from 0.1% TFA. Reconstitute in 10 mM Citric Acid, pH 3.0 to a concentration of 0.1 - 1.0 mg/ml.
• Sequence: Region of TGF beta 1 protein corresponding to amino acids ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
• Sequence Length: 390

• Applicable Applications for TGF beta 1 active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: The three mammalian isoforms of TGF beta, TGF beta1, beta2, beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1.
• Bioactivity: The ED50 was determined by TGF-beta1's ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is approximately 0.05 ng/ml, corresponding to a specific activity of approximately 2 x 10^7 units/mg.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: 293 cells
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for TGF beta 1 active protein

Purified recombinant Human TGF beta 1 protein

Product Categories/Family for TGF beta 1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 50979134
• NCBI GeneID: 403998
• NCBI Accession #: NP_001003309.1
• NCBI GenBank Nucleotide #: NM_001003309.1
• UniProt Accession #: P54831
• Molecular Weight: 44,185 Da
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Symbol: TGFB1
• NCBI Protein Information: transforming growth factor beta-1
• UniProt Protein Name: Transforming growth factor beta-1
• UniProt Gene Name: TGFB1
• UniProt Synonym Gene Names: TGF-beta-1; LAP
• UniProt Entry Name: TGFB1_CANFA

Uniprot Description

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types ().