Human biglycan ELISA Kit | BGN elisa kit

Human Biglycan, BGN ELISA Kit

Cat. Num. AAA937966

• Gene Names: BGN; PGI; DSPG1; PG-S1; SLRR1A
• Reactivity: Human
• Synonyms: biglycan; Human Biglycan; BGN ELISA Kit; Human Biglycan (BGN) ELISA kit; DSPG1; PG-S1; PGI; SLRR1A; biglycan proteoglycan; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; small leucine-rich protein 1A; BGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human BGN. No significant cross-reactivity or interference between human BGN and analogues was observed.
• Sequence Length: 368

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.312 ng/ml -20 ng/ mL
• Sensitivity: <0.078 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BGN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BGN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502403
• NCBI GeneID: 633
• NCBI Accession #: NP_001702.1
• NCBI GenBank Nucleotide #: NM_001711.4
• UniProt Accession #: P21810; P13247; D3DWU3
• Molecular Weight: 41,654 Da
• NCBI Official Full Name: biglycan preproprotein
• NCBI Official Synonym Full Names: biglycan
• NCBI Official Symbol: BGN
• NCBI Official Synonym Symbols: PGI; DSPG1; PG-S1; SLRR1A
• NCBI Protein Information: biglycan; biglycan proteoglycan; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; small leucine-rich protein 1A; dermatan sulphate proteoglycan I
• UniProt Protein Name: Biglycan
• Protein Family: Biglycan
• UniProt Gene Name: BGN
• UniProt Synonym Gene Names: SLRR1A
• UniProt Entry Name: PGS1_HUMAN

NCBI Description

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009]

Uniprot Description

BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma

Molecular Function: glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent

Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; blood vessel remodeling; pathogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process