IL6 active protein

IL6 protein

Cat. Num. AAA538066

• Gene Names: IL6; HGF; HSF; BSF2; IL-6; IFNB2
• Applications: User optimized
• Purity: > 98% pure
• Synonyms: IL6; IL6 protein; HPGF protein; IL-6 protein; IL-6; BCDF protein; 26 kDa protein protein; B cell differentiation factor protein; IFN-lambda2 protein; IL 6 protein; BSF-2 protein; IL 6; Interleukin 6 protein; MGI-2 protein; HSF protein; IL6 active protein

• Host: Human
• Purity/Purification: > 98% pure
• Form/Format: Lyophilized from 10mM Na3PO4, pH 7.5. Reconstitute with distilled water to a concentration of 0.1 - 0.5 mg/ml.
• Sequence: Region of IL6 protein corresponding to amino acids PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM.
• Sequence Length: 212

• Applicable Applications for IL6 active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: IL6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL6 signals through the IL6 receptor system that consists of two chains, IL6R a and gp130. Mouse IL6 is inactive on human cells, while both human and Mouse are equally active on Mouse cells.
• Bioactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6 dependent Mouse 7TD1 cells is approximately 0.1 ng/ml, corresponding to a specific activity of approximately 1 x 10^7units/mg.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for IL6 active protein

Purified recombinant Human IL6 protein

Product Categories/Family for IL6 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 10834984
• NCBI GeneID: 3569
• NCBI Accession #: NP_000591.1
• NCBI GenBank Nucleotide #: NM_000600.3
• UniProt Accession #: P05231; Q9UCU2; Q9UCU3; Q9UCU4
• Molecular Weight: 20.9 kDa
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: HGF; HSF; BSF2; IL-6; IFNB2
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
• UniProt Entry Name: IL6_HUMAN

NCBI Description

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011]

Uniprot Description

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p21

Cellular Component: extracellular space; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; bone remodeling; glucose homeostasis; positive regulation of tyrosine phosphorylation of Stat3 protein; response to caffeine; activation of NF-kappaB transcription factor; response to antibiotic; muscle maintenance; monocyte chemotaxis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; cell growth; response to electrical stimulus; defense response to virus; neurite development; response to drug; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; response to amino acid stimulus; defense response to protozoan; positive regulation of chemokine production; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; response to heat; cell redox homeostasis; positive regulation of B cell activation; neutrophil apoptosis; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; negative regulation of caspase activity; regulation of circadian sleep/wake cycle, non-REM sleep; defense response to Gram-negative bacterium; response to insulin stimulus; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to peptidoglycan; positive regulation of cell proliferation; hepatic immune response; inflammatory response; aging; negative regulation of chemokine biosynthetic process; response to nutrient levels; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; regulation of angiogenesis; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; humoral immune response; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; response to cold; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse

Disease: Rheumatoid Arthritis, Systemic Juvenile; Arteriovenous Malformations Of The Brain; Kaposi Sarcoma, Susceptibility To; Inflammatory Bowel Disease 1