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IFN beta active protein

IFN beta protein

Gene Names
IFNB1; IFB; IFF; IFNB
Applications
User optimized
Purity
> 95% pure
Synonyms
IFN beta; IFN beta protein; Leukocyte interferon; RMMB1; Interferon beta; Type I interferon; B cell interferon; IFN beta active protein
Ordering
For Research Use Only!
Host
Human
Purity/Purification
> 95% pure
Form/Format
Lyophilized from 10mM sodium citrate, with 100mM NaCl, and 10mM Sucrose. Reconstitute in water to a concentration of 0.1-1.0mg/ml. DO NOT VORTEX. For extended storage, dilute further in a buffer containing a carrier protein (ie: 0.1% BSA).
Sequence
Region of IFN Beta protein corresponding to amino acids MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN.
Sequence Length
187
Applicable Applications for IFN beta active protein
User optimized
Protein Type
Recombinant
Biological Significance
Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNa is an acid stable molecule that signals through IFNa/betaR, which is also used by IFNbeta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFNa contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Bioactivity
Assay#1: The biological activity of PeproTech's human IFN-gamma was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activitywas determined to be >5 x 10 8 units/mg, Assay#2: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
CHO cells
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for IFN beta active protein
Purified recombinant Human IFN beta protein
Product Categories/Family for IFN beta active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20 kDa
NCBI Official Full Name
interferon beta
NCBI Official Synonym Full Names
interferon, beta 1, fibroblast
NCBI Official Symbol
IFNB1
NCBI Official Synonym Symbols
IFB; IFF; IFNB
NCBI Protein Information
interferon beta
UniProt Protein Name
Interferon beta
UniProt Gene Name
IFNB1
UniProt Synonym Gene Names
IFB; IFNB; IFN-beta
UniProt Entry Name
IFNB_HUMAN

Uniprot Description

IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; cell surface receptor linked signal transduction; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; B cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

Research Articles on IFN beta

Similar Products

Product Notes

The IFN beta ifnb1 (Catalog #AAA539575) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IFN beta can be used in a range of immunoassay formats including, but not limited to, User optimized. Researchers should empirically determine the suitability of the IFN beta ifnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Region of IFN Beta protein correspond ing to amino acids MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN. It is sometimes possible for the material contained within the vial of "IFN beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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