Follistatin Active Protein | Fst active protein

Follistatin protein

Cat. Num. AAA538118

• Gene Names: Fst; FS; AL033346
• Applications: User optimized
• Purity: > 95% pure
• Synonyms: Follistatin; Follistatin protein; activins-binding protein; FS protein; Fst active protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: Lyophilized from 10mM Na3PO4, pH 7.5, with 75mM NaCl. Reconstitute in distilled water to a concentration of 0.1-1.0 mg/ml
• Sequence: Region of Follistatin protein corresponding to amino acids GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN.
• Sequence Length: 343

• Applicable Applications for Fst active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: Follistatin is a secreted protein that binds to ligands of the TGF beta family and regulates their activity by inhibiting their access to signaling receptors. It was originally discovered as activin antagonists whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle stimulating hormone). In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF beta ligands including BMP-2, -4, -6, -7, Myostatin, GDF-11, and TGF beta1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues.
• Bioactivity: Determined by its ability to neutralize Activin A inhibitory effect of Mouse MPC-11 cells. The expected ED50 is 0.1-0.4 ug/ml in the presence of 7.5 ng/ml Activin A.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for Fst active protein

Purified recombinant Human Follistatin protein

Product Categories/Family for Fst active protein

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 6679867
• NCBI GeneID: 14313
• NCBI Accession #: NP_032072.1
• NCBI GenBank Nucleotide #: NM_008046.2
• UniProt Accession #: P47931; A6H6P0
• Molecular Weight: 31.5 kDa
• NCBI Official Full Name: follistatin isoform 2
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: Fst
• NCBI Official Synonym Symbols: FS; AL033346
• NCBI Protein Information: follistatin
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: Fst
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_MOUSE

NCBI Description

The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH)

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; activin binding

Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; keratinocyte proliferation; pattern specification process; skeletal development; female gonad development; odontogenesis of dentine-containing teeth