Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag Active Protein
Flk-1 Receptor Tyrosine Kinase, Recombinant, Human, N-terminal GST tag (KDR, VEGFR2)
Reactivity
Human
Purity
>90% by SDS-PAGE.
Synonyms
Flk-1 Receptor Tyrosine Kinase; N-terminal GST tag; Recombinant; Human; N-terminal GST tag (KDR; VEGFR2); N-terminal GST tag active protein
Reactivity
Human
Purity/Purification
>90% by SDS-PAGE.
Form/Format
25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
Application Notes
useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity
56 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substarte per minute at pH 7.4 and 30 degree C
Assay Buffer
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.04ug recombinant VEGFR2.
Preparation and Storage
-70 degree C
Stability: >6 months at -80 degree C
Stability: >6 months at -80 degree C
Related Product Information for Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag active protein
Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
Human VEGFR2 GenBank Accession No. AF035121), (a.a. 805-1356) with N-terminal GST tag, MW=87kD, expressed in an Baculovirus infected Sf9 cell expression system..
Human VEGFR2 GenBank Accession No. AF035121), (a.a. 805-1356) with N-terminal GST tag, MW=87kD, expressed in an Baculovirus infected Sf9 cell expression system..
Product Categories/Family for Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag active protein
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Product Notes
The Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag (Catalog #AAA637448) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Flk-1 Receptor Tyrosine Kinase, Recombinant, Human, N-terminal GST tag (KDR, VEGFR2) reacts with Human and may cross-react with other species as described in the data sheet. useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Researchers should empirically determine the suitability of the Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flk-1 Receptor Tyrosine Kinase, N-terminal GST tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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