CD45, aa584-1256, Protein Tyrosine Phosphatase, Catalytic Domain, N-terminal His Tag Active Protein | PTPRC active protein

CD45, aa584-1256, Protein Tyrosine Phosphatase, Catalytic Domain, N-terminal His Tag, Recombinant, Human (Leukocyte Common Antigen, LCA, Ly-5 T200)

Cat. Num. AAA637635

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Purity: >90% by SDS-PAGE.
• Synonyms: CD45; aa584-1256; Protein Tyrosine Phosphatase; Catalytic Domain; N-terminal His Tag; Recombinant; Human (Leukocyte Common Antigen; LCA; Ly-5 T200); PTPRC active protein

• Reactivity: Human
• Purity/Purification: >90% by SDS-PAGE.
• Form/Format: 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 5 mM DTT.

• Application Notes: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
• Specific Activity: 25 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 degree C
• Assay Buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.
• Preparation and Storage: -70 degree C; Stability: >6 months at -80 degree C

Testing Data

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Testing Data

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Related Product Information for PTPRC active protein

Catalytic domain, (a.a. 584-1256) with N-terminal His tag, MW=84.5kD, expressed in a Sf9 baculovirus expression system.

Product Categories/Family for PTPRC active protein

Molecular Biology; MB-Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 44242008
• NCBI GeneID: 5788
• UniProt Accession #: P08575; Q16614; Q9H0Y6; A8K7W6
• Molecular Weight: 147,254 Da
• NCBI Official Full Name: CD45
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of T cell proliferation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To