BD4 active protein

BD4 protein

Cat. Num. AAA539480

• Gene Names: DEFB104A; BD-4; DEFB4; hBD-4; DEFB-4; DEFB104
• Applications: User optimized
• Purity: > 98% pure
• Synonyms: BD4; BD4 protein; BEFB4 protein; BD 4 protein; beta-defensin 104 protein; BD-4; BD-4 protein; BD 4; BD4 active protein

• Host: Human
• Purity/Purification: > 98% pure
• Sequence: Region of BD4 protein corresponding to amino acids EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP.
• Sequence Length: 72

• Applicable Applications for BD4 active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.
• Bioactivity: Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-100.0 ng/ml.
• Endotoxin: Endotoxin level is <0.1 ng/ug of protein (1 EU/ug).
• Expression System: E Coli
• Form: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Acetic Acid. Initially reconstitute in 10mM Acetic Acid to 0.1-1.0mg/mL. After initial reconstitution , further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA)
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for BD4 active protein

Purified recombinant Human BD4 protein

Product Categories/Family for BD4 active protein

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 103485139
• NCBI GeneID: 140596
• NCBI Accession #: NP_525128.2
• NCBI GenBank Nucleotide #: NM_080389.2
• UniProt Accession #: Q8WTQ1; Q496I2; Q496I3; Q496I4
• Molecular Weight: 6.0 kDa
• NCBI Official Full Name: beta-defensin 104
• NCBI Official Synonym Full Names: defensin, beta 104A
• NCBI Official Symbol: DEFB104A
• NCBI Official Synonym Symbols: BD-4; DEFB4; hBD-4; DEFB-4; DEFB104
• NCBI Protein Information: beta-defensin 104
• UniProt Protein Name: Beta-defensin 104
• UniProt Gene Name: DEFB104A
• UniProt Synonym Gene Names: DEFB104; DEFB4; BD-4; DEFB-4; hBD-4
• UniProt Entry Name: D104A_HUMAN

NCBI Description

Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, beta 4: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Belongs to the beta-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cell surface; extracellular region

Biological Process: defense response to bacterium; innate immune response