Angiostatin Kringles 1-3 Active Protein
Angiostatin Kringles 1-3, Human
Applications
ELISA
Purity
Highly Purified>95% (SDS-PAGE). Free from plasmin or plasminogen.
Synonyms
Angiostatin Kringles 1-3; Human; Angiostatin Kringles 1-3 active protein
Host
Human
Purity/Purification
Highly Purified
>95% (SDS-PAGE). Free from plasmin or plasminogen.
>95% (SDS-PAGE). Free from plasmin or plasminogen.
Form/Format
Supplied as a liquid in PBS.
Applicable Applications for Angiostatin Kringles 1-3 active protein
ELISA (EL/EIA)
Activity
Biologically active. Inhibitory activity (ED50 =70nM) relative to Kringles 1-4 (ED50 =135nM)
Storage and Stability:
Aliquot and store at -70 degree C. Stable for 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Storage and Stability:
Aliquot and store at -70 degree C. Stable for 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Preparation and Storage
-70 degree C
Related Product Information for Angiostatin Kringles 1-3 active protein
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. Angiostatin protein, a proteolytic fragment of plasminogen, is comprised of the first four kringle regions. It prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Kringles 1-3 fragment has a greater inhibitory activity than the Kringles 1-4 fragment. The protease-activated Kringles 1-5 is the most potent plasminogen fragment with over 50-fold greater endothelial cell specific inhibitory activity. Its systemic administration inhibited the growth of fibrosarcoma and significantly reduced neovascularization.
Purified human Kringles 1-3 (MW ~38kD) is prepared from human fluids negative for HBsAg, HIV, and HCV.
Recent studies have identified several proteolytic fragments or cryptic domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein (kringles 1-4) and kringles 1-5, C-terminal proteolytic fragment of Collagen XVIII (Endostatin protein), the NC10 domain of collagen 15 (Restin), the C-terminal hemopexin-like domain of MMP-2 (PEX), the N-terminal fragment of prolactin, and the N-terminally truncated platelet factor. Angiostatin protein, a proteolytic fragment of plasminogen, is comprised of the first four kringle regions. It prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Kringles 1-3 fragment has a greater inhibitory activity than the Kringles 1-4 fragment. The protease-activated Kringles 1-5 is the most potent plasminogen fragment with over 50-fold greater endothelial cell specific inhibitory activity. Its systemic administration inhibited the growth of fibrosarcoma and significantly reduced neovascularization.
Purified human Kringles 1-3 (MW ~38kD) is prepared from human fluids negative for HBsAg, HIV, and HCV.
Product Categories/Family for Angiostatin Kringles 1-3 active protein
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Product Notes
The Angiostatin Kringles 1-3 (Catalog #AAA634664) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Angiostatin Kringles 1-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Researchers should empirically determine the suitability of the Angiostatin Kringles 1-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiostatin Kringles 1-3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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