Sheep Histatin 5 ELISA Kit | HTN5 elisa kit

Sheep Histatin 5 ELISA Kit

Cat. Num. AAA747147

• Gene Names: HTN3; HIS2; HTN2; HTN5
• Reactivity: Sheep
• Synonyms: Histatin 5; Sheep Histatin 5 ELISA Kit; HTN5 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This HTN5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep HTN5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTN5 elisa kit

Principle of the assay: HTN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HTN5 antibody and an HTN5-HRP conjugate. The assay sample and buffer are incubated together with HTN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HTN5 concentration since HTN5 from samples and HTN5-HRP conjugate compete for the anti-HTN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by HTN5 from the sample, fewer sites are left to bind HTN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HTN5 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557653
• NCBI GeneID: 3347
• NCBI Accession #: NP_000191.1
• NCBI GenBank Nucleotide #: NM_000200.2
• Molecular Weight: 6,149 Da
• NCBI Official Full Name: histatin-3
• NCBI Official Synonym Full Names: histatin 3
• NCBI Official Symbol: HTN3
• NCBI Official Synonym Symbols: HIS2; HTN2; HTN5
• NCBI Protein Information: histatin-3; PB; hst; histatin 5; histatin-4; histatin-5; histatin-6; histatin-7; histatin-8; histatin-9; histidine-rich protein 3; basic histidine-rich protein
• UniProt Protein Name: Histatin-3
• UniProt Gene Name: HTN3
• UniProt Synonym Gene Names: HIS2; Hst; His3 20/44; His3 1/25; His3 20/43; His3 1/24; His3 20/32; His3 1/13; His3 20/31; His3 1/12; His3 20/30; His3 1/11; His3 24/32; His3 5/13; His3 24/31; His3 5/12; His3 24/30; His3 5/11; His3 25/32; His3 6/13; His3 25/30; His3 6/11; His3 26/32; Hi
• UniProt Entry Name: HIS3_HUMAN

NCBI Description

This gene encodes a member of the histatin family of small, histidine-rich, cationic proteins. They function as antimicrobial peptides and are important components of the innate immune system. Histatins are found in saliva and exhibit antibacterial, antifungal activities and function in wound healing. [provided by RefSeq, Sep 2014]

Uniprot Description

HTN3: Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. His3-(20-43)-peptide (histatin-5) is especially effective against C.albicans and C.neoformans, and inhibits Lys-gingipain and Arg-gingipain (rgpB) from P.gingivalis. In addition, His3-(20-43)-peptide is a potent inhibitor of metalloproteinases MMP2 and MMP9. Belongs to the histatin/statherin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: extracellular region

Molecular Function: protein binding; metal ion binding

Biological Process: killing of cells of another organism; biomineral formation; defense response to bacterium; defense response to fungus