Heat Shock 10kDa Protein 1 (HSP10) Recombinant Protein | HSP10 recombinant protein

Recombinant Heat Shock 10kDa Protein 1 (HSP10)

Cat. Num. AAA2012059

• Gene Names: HSPE1; EPF; CPN10; GROES; HSP10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock 10kDa Protein 1 (HSP10); Recombinant Heat Shock 10kDa Protein 1 (HSP10); HSP10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-AGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD
• Sequence Length: 102

• Applicable Applications for HSP10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala2~Asp102 (Accession # P61604) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4504523
• NCBI GeneID: 3336
• NCBI Accession #: NP_002148.1
• NCBI GenBank Nucleotide #: NM_002157.2
• UniProt Accession #: P61604; O95421; Q04984; Q53X54; Q9UDH0
• Molecular Weight: 12.3kDa
• NCBI Official Full Name: 10 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 10kDa protein 1
• NCBI Official Symbol: HSPE1
• NCBI Official Synonym Symbols: EPF; CPN10; GROES; HSP10
• NCBI Protein Information: 10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
• UniProt Protein Name: 10 kDa heat shock protein, mitochondrial
• Protein Family: 10 kDa heat shock protein
• UniProt Gene Name: HSPE1
• UniProt Synonym Gene Names: Hsp10; CPN10; EPF
• UniProt Entry Name: CH10_HUMAN

NCBI Description

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Uniprot Description

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrion; mitochondrial matrix

Molecular Function: protein binding; chaperone binding; unfolded protein binding; ATP binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein