Typical Testing Data/Standard Curve (for reference only)
Rat heat shock 27kDa protein 1 ELISA Kit | HSPB1 elisa kit
Rat heat shock protein 27, HSP-27 ELISA Kit
Gene Names
Hspb1; Hsp25; Hsp27
Reactivity
Rat
Synonyms
heat shock 27kDa protein 1; Rat heat shock protein 27; HSP-27 ELISA Kit; CMT2F; DKFZp586P1322; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27; estrogen-regulated 24 kDa protein; heat shock 27kD protein 1; heat shock protein beta-1; stress-responsive protein 27; HSPB1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat HSPB1. No significant cross-reactivity or interference between Rat HSPB1 and analogues was observed.
Sequence Length
205
Samples
Serum, plasma, tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml
Sensitivity
0.195 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Neurobiology
Protein Biological Process 3
Stress response
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSPB1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated withan antibody specific to HSP-27. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for HSP-27 andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain HSP-27, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of HSP-27 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,893 Da
NCBI Official Full Name
heat shock protein beta-1
NCBI Official Synonym Full Names
heat shock protein B1
NCBI Official Symbol
Hspb1
NCBI Official Synonym Symbols
Hsp25; Hsp27
NCBI Protein Information
heat shock protein beta-1; HSP 27; heat shock protein 1; heat shock 27kDa protein 1; heat shock 27 kDa protein 1
UniProt Protein Name
Heat shock protein beta-1
Protein Family
UniProt Gene Name
Hspb1
UniProt Synonym Gene Names
Hsp27; HspB1; HSP 27
UniProt Entry Name
HSPB1_RAT
NCBI Description
putative heat shock protein; human homolog may suppress polyglutamine-mediated cell death and mutations in human gene are associated with various neuropathies and some forms of Charcot-Marie-Tooth disease [RGD, Feb 2006]
Uniprot Description
Function: Involved in stress resistance and actin organization.
Research Articles on HSPB1
Similar Products
Product Notes
The Rat HSPB1 hspb1 (Catalog #AAA700121) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700121 ELISA Kit recognizes Rat HSPB1. It is sometimes possible for the material contained within the vial of "heat shock 27kDa protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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