Pig Heat shock protein beta-1 ELISA Kit | HSPB1 elisa kit
Pig Heat shock protein beta-1 (HSPB1) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HSP-70 in Pig serum, plasma. An antibody specific for HSP-70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-70 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-70 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
Protein type: Chaperone; Heat shock protein; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; extracellular matrix; focal adhesion; nucleus; plasma membrane; spindle; Z disc
Molecular Function: identical protein binding; protein homodimerization activity; protein kinase C binding; protein kinase C inhibitor activity; RNA binding
Biological Process: positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of protein amino acid phosphorylation; response to virus; retinal homeostasis
Research Articles on HSPB1
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Product Notes
The Pig HSPB1 hspb1 (Catalog #AAA283510) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283510 ELISA Kit recognizes Pig HSPB1. It is sometimes possible for the material contained within the vial of "Heat shock protein beta-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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