SDS-PAGE
Mortalin Recombinant Protein | HSPA9 recombinant protein
Mortalin recombinant protein
Gene Names
HSPA9; CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
Applications
Western Blot
Synonyms
Mortalin; Mortalin recombinant protein; HSPA9; CSA; GRP75; HSPA9B; MGC4500; MOT; mot-2; MOT2; MTHSP75; PBP74; HSPA9 recombinant protein
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
3506
Applicable Applications for HSPA9 recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human Mortalin (47-end) protein was expressed in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for HSPA9 recombinant protein
Recombinant human Mortalin (47-end) protein was expressed in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Mortalin is a mitochondrial chaperone and a member of the heat shock protein 70 family that is constitutively expressed in cells. Mortalin plays a central role in mitochondrial biogenesis through its capacity to direct the import of nuclear-encoded proteins into the mitochondria. Mortalin plays a role in the control of cell proliferation and elevated levels of mortalin have correlated with malignant transformation and poor cancer prognosis. Mortalin can support cancer cell resistance to complement-dependent cytotoxicity (1). Mortalin expression was decreased in the mitochondrial fraction of neurons from the substantia nigra of Parkinson disease patients (2).
Scientific Background: Mortalin is a mitochondrial chaperone and a member of the heat shock protein 70 family that is constitutively expressed in cells. Mortalin plays a central role in mitochondrial biogenesis through its capacity to direct the import of nuclear-encoded proteins into the mitochondria. Mortalin plays a role in the control of cell proliferation and elevated levels of mortalin have correlated with malignant transformation and poor cancer prognosis. Mortalin can support cancer cell resistance to complement-dependent cytotoxicity (1). Mortalin expression was decreased in the mitochondrial fraction of neurons from the substantia nigra of Parkinson disease patients (2).
Product Categories/Family for HSPA9 recombinant protein
References
1. Pilzer, D. et al: Mortalin inhibitors sensitize K562 leukemia cells to complement-dependent cytotoxicity. Int J Cancer. 2010 Mar 15;126(6):1428-35.
2. De Mena, L. et al: Mutational screening of the mortalin gene (HSPA9) in Parkinson's disease. J Neural Transm. 2009 Oct;116(10):1289-93.
2. De Mena, L. et al: Mutational screening of the mortalin gene (HSPA9) in Parkinson's disease. J Neural Transm. 2009 Oct;116(10):1289-93.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~97 kDa
NCBI Official Full Name
Homo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9), mRNA
NCBI Official Synonym Full Names
heat shock 70kDa protein 9 (mortalin)
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75
NCBI Protein Information
stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein
UniProt Protein Name
Stress-70 protein, mitochondrial
Protein Family
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN
NCBI Description
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]
Uniprot Description
Function: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Subunit structure: Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Ref.13 Ref.15 Ref.18 Ref.20
Subcellular location: Mitochondrion. Nucleus › nucleolus Ref.19.
Sequence similarities: Belongs to the heat shock protein 70 family.
Research Articles on HSPA9
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Product Notes
The HSPA9 hspa9 (Catalog #AAA515177) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human Mortalin (47-end) protein was expressed in Sf9 insect cells. AAA Biotech's Mortalin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HSPA9 hspa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mortalin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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