HSC70/HSP73 recombinant protein

HSC70/HSP73 (bovine), (recombinant)

Cat. Num. AAA565960

• Gene Names: HSPA8; Hsc70; HSPA10
• Applications: Western Blot
• Purity: >=90% (SDS-PAGE; Western blot); Purified by multi-step chromatography
• Synonyms: HSC70/HSP73; HSC70/HSP73 (bovine); (recombinant); HSC70/HSP73 recombinant protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE; Western blot); Purified by multi-step chromatography
• Form/Format: Liquid. In 30mM TRIS-HCl, pH7.5, containing 1.0mM EDTA, 2.0mM DTT, and 300mM sodium chloride

• Applicable Applications for HSC70/HSP73 recombinant protein: Western Blot (WB), Activity assay
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for HSC70/HSP73 recombinant protein

The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Categories/Family for HSC70/HSP73 recombinant protein

Proteostasis; Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 76253709
• NCBI GeneID: 281831
• NCBI Accession #: NP_776770.2
• NCBI GenBank Nucleotide #: NM_174345.4
• UniProt Accession #: P19120; Q3MHM4; A5D968
• Molecular Weight: 70kD
• NCBI Official Full Name: heat shock cognate 71 kDa protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: Hsc70; HSPA10
• NCBI Protein Information: heat shock cognate 71 kDa protein; Hsc70 ATPase; heat shock 70 kDa protein 8; heat shock cognate 71 kD protein; heat shock 70kd protein 10 (HSC71)
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: HSPA8
• UniProt Synonym Gene Names: HSC70
• UniProt Entry Name: HSP7C_BOVIN

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; cell surface; focal adhesion; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus; myelin sheath

Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; unfolded protein binding; ribosome binding; misfolded protein binding; glycoprotein binding; ATP binding

Biological Process: cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cerebellum structural organization; substantia nigra development; proteolysis involved in cellular protein catabolic process; positive regulation of protein ubiquitination; cerebellar Purkinje cell layer development; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis; positive regulation of cell migration