Typical Testing Data/Standard Curve (for reference only)
Mouse Heat Shock 70kDa Protein 4 ELISA Kit | HSPA4 elisa kit
Mouse Heat Shock 70kDa Protein 4 ELISA Kit
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Reactivity
Mouse
Synonyms
Heat Shock 70kDa Protein 4; Mouse Heat Shock 70kDa Protein 4 ELISA Kit; HSPA4 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSPA4 elisa kit
Intended Uses: This HSPA4 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHSPA4 in the sample, thisHSPA4 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHSPA4 concentration. The concentration ofHSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: This HSPA4 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHSPA4. Standards or samples are then added to the microtiter plate wells andHSPA4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHSPA4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHSPA4 are added to each well to "sandwich" theHSPA4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHSPA4 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Principle of the Assay: This HSPA4 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHSPA4. Standards or samples are then added to the microtiter plate wells andHSPA4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHSPA4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHSPA4 are added to each well to "sandwich" theHSPA4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHSPA4 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Product Categories/Family for HSPA4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,331 Da
NCBI Official Full Name
heat shock 70 kDa protein 4
NCBI Official Synonym Full Names
heat shock 70kDa protein 4
NCBI Official Symbol
HSPA4
NCBI Official Synonym Symbols
RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
NCBI Protein Information
heat shock 70 kDa protein 4; hsp70 RY; heat shock 70kD protein 4; heat shock protein, 110 kDa; heat shock 70-related protein APG-2; epididymis secretory sperm binding protein Li 5a
UniProt Protein Name
Heat shock 70 kDa protein 4
Protein Family
UniProt Gene Name
HSPA4
UniProt Synonym Gene Names
APG2
UniProt Entry Name
HSP74_HUMAN
Uniprot Description
HSP70RY: Interacts with TJP1/ZO-1. Belongs to the heat shock protein 70 family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: mitochondrion; cytosol
Molecular Function: ATP binding
Biological Process: chaperone-mediated protein complex assembly; protein import into mitochondrial outer membrane; response to unfolded protein
Research Articles on HSPA4
Similar Products
Product Notes
The Mouse HSPA4 hspa4 (Catalog #AAA727929) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727929 ELISA Kit recognizes Mouse HSPA4. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
