Human Endoplasmin ELISA Kit | HSP90B1 elisa kit

Human Endoplasmin ELISA Kit

Cat. Num. AAA762230

• Gene Names: HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• Reactivity: Human
• Synonyms: Endoplasmin; Human Endoplasmin ELISA Kit; HSP90B1/gp96/ECGP/Endoplasmin/Grp94/94 kDa glucose-regulated protein/endothelial cell (HBMEC) glycoprotein/glucose regulated protein; 94 kDa/GP96ECGP/GRP-94/GRP94gp96 homolog/Heat shock protein 90 kDa beta member 1/heat shock protein 90kDa beta (Grp94); member 1; HSP90B1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HSP90B1. No significant cross-reactivity or interference between HSP90B1 and analogues was observed.
• Sequence Length: 803

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP90B1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507677
• NCBI GeneID: 7184
• NCBI Accession #: NP_003290.1
• NCBI GenBank Nucleotide #: NM_003299.2
• Molecular Weight: 92,469 Da
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90kDa beta family member 1
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_HUMAN

NCBI Description

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Apoptosis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q24.2-q24.3

Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; extracellular matrix; extracellular region; focal adhesion; melanosome; membrane; midbody; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; calcium ion binding; low-density lipoprotein receptor binding; protein binding; protein phosphatase binding; RNA binding; unfolded protein binding; virion binding

Biological Process: actin rod formation; cellular protein metabolic process; ER-associated protein catabolic process; innate immune response; negative regulation of apoptosis; protein transport; receptor-mediated endocytosis; regulation of phosphoprotein phosphatase activity; response to hypoxia; retrograde protein transport, ER to cytosol; sequestering of calcium ion; toll-like receptor signaling pathway; unfolded protein response