Hsp104 recombinant protein

Hsp104, 1-908aa, Saccharomyces cerevisiae, E Coli

Cat. Num. AAA203033

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Hsp104; 1-908aa; Saccharomyces cerevisiae; E Coli; Heat shock protein 104; Heat shock protein 104 L0948; Hsp 104; Hsp104 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 2 mM EDTA, 5% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MNDQTQFTER ALTILTLAQK LASDHQHPQL QPIHILAAFI ETPEDGSVPY LQNLIEKGRY DYDLFKKVVN RNLVRIPQQQ PAPAEITPSY ALGKVLQDAA KIQKQQKDSF IAQDHILFAL FNDSSIQQIF KEAQVDIEAI KQQALELRGN TRIDSRGADT NTPLEYLSKY AIDMTEQARQ GKLDPVIGRE EEIRSTIRVL ARRIKSNPCL IGEPGIGKTA IIEGVAQRII DDDVPTILQG AKLFSLDLAA LTAGAKYKGD FEERFKGVLK EIEESKTLIV LFIDEIHMLM GNGKDDAANI LKPALSRGQL KVIGATTNNE YRSIVEKDGA FERRFQKIEV AEPSVRQTVA ILRGLQPKYE IHHGVRILDS ALVTAAQLAK RYLPYRRLPD SALDLVDISC AGVAVARDSK PEELDSKERQ LQLIQVEIKA LERDEDADST TKDRLKLARQ KEASLQEELE PLRQRYNEEK HGHEELTQAK KKLDELENKA LDAERRYDTA TAADLRYFAI PDIKKQIEKL EDQVAEEERR AGANSMIQNV VDSDTISETA ARLTGIPVKK LSESENEKLI HMERDLSSEV VGQMDAIKAV SNAVRLSRSG LANPRQPASF LFLGLSGSGK TELAKKVAGF LFNDEDMMIR VDCSELSEKY AVSKLLGTTA GYVGYDEGGF LTNQLQYKPY SVLLFDEVEK AHPDVLTVML QMLDDGRITS GQGKTIDCSN CIVIMTSNLG AEFINSQQGS KIQESTKNLV MGAVRQHFRP EFLNRISSIV IFNKLSRKAI HKIVDIRLKE IEERFEQNDK HYKLNLTQEA KDFLAKYGYS DDMGARPLNR LIQNEILNKL ALRILKNEIK DKETVNVVLK KGKSRDENVP EEAEECLEVL PNHEATIGAD TLGDDDNEDS MEIDDDLD
• Sequence Length: 908

• Applicable Applications for Hsp104 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Hsp104 recombinant protein

Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi (+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Product Categories/Family for Hsp104 recombinant protein

Heat Shock Proteins

References

Abbas-Terki T et al, (2001) Mol Cell Biol, 21: 7569-7575.; Sanchez Y et al, (1990) Science, 248: 1112-1115.; Parsell DA et al, (1991) Nature, 353: 270-273.;

NCBI and Uniprot Product Information

• NCBI GI #: 6323002
• NCBI GeneID: 850633
• NCBI Accession #: NP_013074
• NCBI GenBank Nucleotide #: NM_001181846.1
• UniProt Accession #: P31539; D6VXX8
• Molecular Weight: 102kDa (908aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: chaperone ATPase HSP104
• NCBI Official Symbol: HSP104
• NCBI Protein Information: chaperone ATPase HSP104
• UniProt Protein Name: Heat shock protein 104
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP104

Uniprot Description

Required, in concert with Hsp40 (YDJ1) and Hsp70 (SSA1) and small Hsps (HSP26), for the dissociation, resolubilization and refolding of aggregates of damaged proteins after heat or other environmental stresses. Extracts proteins from aggregates by unfolding and threading them in an ATP-dependent process through the axial channel of the protein hexamer, after which they can be refolded by components of the Hsp70/Hsp40 chaperone system. Substrate binding is ATP-dependent, and release of bound polypeptide is triggered by ATP hydrolysis. Also responsible for the maintenance of prions by dissociating prion fibrils into smaller oligomers, thereby producing transmissible seeds that can infect daughter cells during mitosis and meiosis. Loss of HSP104 can cure yeast cells of the prions [PSI+], [URE3] and [PIN+]. Excess HSP104 can also specifically cure cells of [PSI+].