Pig Heat Shock Protein 70 ELISA Kit | HSP-70 elisa kit
Pig Heat Shock Protein 70 (HSP-70) ELISA Kit
Gene Names
AT5G02490; T22P11.80; T22P11_80
Reactivity
Pig
Synonyms
Heat Shock Protein 70; Pig Heat Shock Protein 70 (HSP-70) ELISA Kit; HSP-70 elisa kit
Reactivity
Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig GYPA. No significant cross-reactivity or interference between Pig GYPA and analogues was observed.
Sequence Length
653
Samples
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
10.4 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HSP-70 elisa kit
Intended Uses: For the quantitative detection of Pig Glycophorin-A (GYPA) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GYPA in Pig serum, plasma, tissue homogenates. An antibody specific for GYPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GYPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GYPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GYPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GYPA in Pig serum, plasma, tissue homogenates. An antibody specific for GYPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GYPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GYPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GYPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,387 Da
NCBI Official Full Name
heat shock protein 70
NCBI Official Symbol
AT5G02490
NCBI Official Synonym Symbols
T22P11.80; T22P11_80
NCBI Protein Information
heat shock protein 70
UniProt Protein Name
Probable mediator of RNA polymerase II transcription subunit 37c
Protein Family
UniProt Gene Name
MED37D
UniProt Synonym Gene Names
HSC70-2; HSC70-G8; HSP70-2; MED37_3; AtHsc70-2; AtHsp70-2
UniProt Entry Name
MD37D_ARATH
Uniprot Description
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors ().
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Product Notes
The Pig HSP-70 med37d (Catalog #AAA283326) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283326 ELISA Kit recognizes Pig HSP-70. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 70, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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