Mouse Heat shock factor protein 1 ELISA Kit | Hsf1 elisa kit

Mouse Heat shock factor protein 1 ELISA Kit

Cat. Num. AAA2884282

• Gene Names: Hsf1; AA960185; Hsf1beta; Hsf1alpha
• Reactivity: Mouse
• Synonyms: Heat shock factor protein 1; Mouse Heat shock factor protein 1 ELISA Kit; HSF 1; Heat shock transcription factor 1; HSTF 1; Hsf1; Hsf1 elisa kit

• Reactivity: Mouse
• Sequence Length: 503

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/mL
• Sensitivity: 0.158 ng/mL
• Intra-Assay CV: <=8.7%
• Inter-Assay CV: <=10.1%
• Recovery: 102%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 33859480
• NCBI GeneID: 15499
• NCBI Accession #: NP_032322.1
• NCBI GenBank Nucleotide #: NM_008296.2
• UniProt Accession #: P38532; O70462
• Molecular Weight: 54,931 Da
• NCBI Official Full Name: heat shock factor protein 1
• NCBI Official Synonym Full Names: heat shock factor 1
• NCBI Official Symbol: Hsf1
• NCBI Official Synonym Symbols: AA960185; Hsf1beta; Hsf1alpha
• NCBI Protein Information: heat shock factor protein 1
• UniProt Protein Name: Heat shock factor protein 1
• Protein Family: Heat shock factor protein
• UniProt Gene Name: Hsf1
• UniProt Synonym Gene Names: HSF 1; HSTF 1
• UniProt Entry Name: HSF1_MOUSE

Uniprot Description

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Cellular Component: cytoplasm; euchromatin; heterochromatin; nucleoplasm; nucleus; pronucleus; protein complex

Molecular Function: chromatin binding; DNA binding; heat shock protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: defense response; embryonic placenta development; embryonic process involved in female pregnancy; female meiosis; in utero embryonic development; mRNA transcription; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of caspase activity; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; response to estradiol stimulus; response to heat; response to lipopolysaccharide; response to testosterone stimulus; spermatogenesis; transcription, DNA-dependent