Mouse Heparanase ELISA Kit | HPA/Hpse elisa kit

Mouse Heparanase ELISA Kit

Cat. Num. AAA760453

• Gene Names: HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
• Reactivity: Mouse
• Synonyms: Heparanase; Mouse Heparanase ELISA Kit; HPA/Hpse (Heparanase)/HEP/HPA/HPA1/HPR1/HPSE1/HSE1/Endo-glucoronidase/Heparanase-1; HPA/Hpse elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of HPA/Hpse. No significant cross-reactivity or interference between HPA/Hpse and analogues was observed.
• Sequence Length: 543

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: <46.875pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HPA/Hpse were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HPA/Hpse were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for HPA/Hpse elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HPA/Hpse antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HPA/Hpse antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HPA/Hpse amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HPA/Hpse can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5880863
• NCBI GeneID: 10855
• Molecular Weight: 42,791 Da
• NCBI Official Full Name: heparanase
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: HPSE
• NCBI Official Synonym Symbols: HPA; HPA1; HPR1; HSE1; HPSE1
• NCBI Protein Information: heparanase
• UniProt Protein Name: Heparanase
• Protein Family: Heparanase
• UniProt Gene Name: HPSE
• UniProt Synonym Gene Names: HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1
• UniProt Entry Name: HPSE_HUMAN

NCBI Description

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: Secreted; Secreted, signal peptide; Glycan Metabolism - glycosaminoglycan degradation; EC 3.2.1.166; Extracellular matrix; Hydrolase

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: extracellular region; intracellular membrane-bound organelle; lipid raft; lysosomal lumen; lysosomal membrane; lysosome; nucleoplasm; nucleus

Molecular Function: beta-glucuronidase activity; heparanase activity; protein binding; protein dimerization activity; syndecan binding

Biological Process: carbohydrate metabolic process; cell-matrix adhesion; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan catabolic process; positive regulation of blood coagulation; positive regulation of hair follicle development; positive regulation of osteoblast proliferation; positive regulation of protein kinase B signaling cascade; proteoglycan metabolic process; regulation of hair follicle development