Horse TAFI (Thrombin Acivatable Fibrinolysis Inhibitor) ELISA Kit | TAFI elisa kit

Horse TAFI (Thrombin Acivatable Fibrinolysis Inhibitor)

Cat. Num. AAA766165

• Gene Names: Cpb2; CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• Reactivity: Horse
• Synonyms: TAFI (Thrombin Acivatable Fibrinolysis Inhibitor); Horse TAFI (Thrombin Acivatable Fibrinolysis Inhibitor); TAFI elisa kit

• Reactivity: Horse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TAFI . No significant cross-reactivity or interference between TAFI and analogues was observed.
• Sequence Length: 422

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 3.125-200ng/ml
• Sensitivity: 1.875ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TAFI were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TAFI were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAFI elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TAFI antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TAFI antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TAFI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TAFI can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7416967
• NCBI GeneID: 56373
• Molecular Weight: 48,871 Da
• NCBI Official Full Name: thrombin-activatable fibrinolysis inhibitor
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: Cpb2
• NCBI Official Synonym Symbols: CPR; Cpu; TAFI; AI255929; 1110032P04Rik; 4930405E17Rik
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• UniProt Gene Name: Cpb2
• UniProt Synonym Gene Names: Tafi; CPR; CPU; TAFI
• UniProt Entry Name: CBPB2_MOUSE

NCBI Description

This gene encodes carboxypeptidase B, a zinc-dependent metalloprotease that cleaves peptide bonds at the C-terminus of protein substrates. The encoded preproprotein undergoes proteolytic activation to generate a mature, functional enzyme, and secreted into plasma. [provided by RefSeq, Jan 2016]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.20; Secreted, signal peptide; Secreted; Protease

Cellular Component: extracellular region; extracellular space

Molecular Function: carboxypeptidase activity; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Biological Process: blood coagulation; fibrinolysis; hemostasis; negative regulation of fibrinolysis; proteolysis; response to heat