Hamster Heme Oxygenase 1, Decycling ELISA Kit | HO1 elisa kit

Hamster Heme Oxygenase 1, Decycling ELISA Kit

Cat. Num. AAA34959

• Gene Names: HMOX1; HO-1; HSP32; HMOX1D; bK286B10
• Reactivity: Hamster
• Synonyms: Heme Oxygenase 1; Decycling; Hamster Heme Oxygenase 1; Decycling ELISA Kit; HO1 elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster HO1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-20ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated HO1 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster HO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HO1 and HO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for HO1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated HO1 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster HO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HO1 and HO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504437
• NCBI GeneID: 3162
• NCBI Accession #: NP_002124.1
• NCBI GenBank Nucleotide #: NM_002133.2
• UniProt Accession #: P09601
• Molecular Weight: 32,819 Da
• NCBI Official Full Name: heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 1
• NCBI Official Symbol: HMOX1
• NCBI Official Synonym Symbols: HO-1; HSP32; HMOX1D; bK286B10
• NCBI Protein Information: heme oxygenase 1; heat shock protein, 32-kD
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: HMOX1
• UniProt Synonym Gene Names: HO; HO1; HO-1
• UniProt Entry Name: HMOX1_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Uniprot Description

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity

Biological Process: response to nicotine; cell death; cellular iron ion homeostasis; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; excretion; positive regulation of vasodilation; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; small GTPase mediated signal transduction; regulation of blood pressure; porphyrin metabolic process; negative regulation of mast cell cytokine production; negative regulation of neuron apoptosis; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; transmembrane transport; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; iron ion homeostasis; positive regulation of angiogenesis; positive regulation of chemokine biosynthetic process; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia

Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive