DYRK1B recombinant protein

DYRK1B, Active

Cat. Num. AAA516780

• Gene Names: DYRK1B; MIRK; AOMS3
• Applications: Western Blot
• Synonyms: DYRK1B; Active; MIRK; DYRK1B recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for DYRK1B recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for DYRK1B recombinant protein

Recombinant full-length human DYRK1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC018751.
DYRK1B (Dual specificity tyrosine-phosphorylation-regulated kinase 1B) is a member of the evolutionarily conserved DYRK family with key roles in the control of cell proliferation and differentiation. DYRK1B acts in G0/G1 to maintain cells in growth arrest and quiescence by targeting cyclin D1 for proteasomal degradation by phosphorylating a threonine residue close to the C terminus. Paradoxically, DYRK1B can also stabilize other proteins by phosphorylation as illustrated by the phosphorylation by DYRK1B of p27Kip1 on Ser10 during the G0 phase of the cell cycle. Deregulation of DYRK1B such as gene amplification, overexpression and/or constitutive activation has been observed in multiple types of cancer.

Product Categories/Family for DYRK1B recombinant protein

Protein Kinases; AKT/PKB Pathway; Apoptosis/Autophagy; Cancer; Cardiovascular Disease; Inflammation; Invasion/Metastasis; Metabolic Disorder; Neurobiology; NfkB Pathway; Ser/Thr Kinases; WNT Signaling

References

1. Becker W. Emerging role of DYRK family protein kinases as regulators of protein stability in cell cycle control. Cell Cycle. 2012 11:3389-94.
2. Deng X, Mercer SE, Shah S, Ewton DZ, Friedman E. The cyclin-dependent kinase inhibitor p27Kip1 is stabilized in G(0) by Mirk/dyrk1B kinase. J Biol Chem. 2004 279:22498-504.

NCBI and Uniprot Product Information

• NCBI GI #: 17511802
• NCBI GeneID: 9149
• Molecular Weight: 106.0 kDa
• NCBI Official Full Name: Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B, mRNA
• NCBI Official Synonym Full Names: dual specificity tyrosine phosphorylation regulated kinase 1B
• NCBI Official Symbol: DYRK1B
• NCBI Official Synonym Symbols: MIRK; AOMS3
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1B
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase

NCBI Description

This gene encodes a member of a family of nuclear-localized protein kinases. The encoded protein participates in the regulation of the cell cycle. Expression of this gene may be altered in tumor cells, and mutations in this gene were found to cause abdominal obesity-metabolic syndrome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]