SDS-Page
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
HMGN1 recombinant protein
HMGN1, 1-100aa, Human, His tag, E Coli
Gene Names
HMGN1; HMG14
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HMGN1; 1-100aa; Human; His tag; E Coli; Non-histone chromosomal protein HMG-14; HMG14; GC104230; Non-histone chromosomal protein HMG-14 FLJ27265; FLJ31471; High mobility group (nonhistone chromosomal) protein 14; High mobility group nucleosome binding 1; High mobility group nucleosome binding domain 1; High mobility group nucleosome binding domain containing protein 1; High mobility group protein 14; HMG 14 HMGN 1; MGC104230; MGC117425; Nonhistone chromosomal protein HMG 14; Nonhistone chromosomal protein HMG14.; HMGN1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 0.1mM PMSF
Concentration
0.5 mg/ml (determined by BCA assay) (varies by lot)
Sequence
MPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD LEHHHHHH
Sequence Length
100
Applicable Applications for HMGN1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at -2°C to -8°C.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for HMGN1 recombinant protein
HMGN1, also known as non-histone chromosomal protein HMG-14, belongs to the HMGN family. Chromosomal protein HMGN1 binds to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. This protein may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Recombinant human HMGN1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HMGN1 recombinant protein
References
Landsman D. et al, (1989) J Biol Chem. 264 (6):3421-7
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11.7 kDa (108aa), confirmed by MALDI-TOF.
NCBI Official Full Name
non-histone chromosomal protein HMG-14
NCBI Official Synonym Full Names
high mobility group nucleosome binding domain 1
NCBI Official Symbol
HMGN1
NCBI Official Synonym Symbols
HMG14
NCBI Protein Information
non-histone chromosomal protein HMG-14
UniProt Protein Name
Non-histone chromosomal protein HMG-14
Protein Family
UniProt Gene Name
HMGN1
UniProt Synonym Gene Names
HMG14
NCBI Description
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]
Uniprot Description
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 ().
Research Articles on HMGN1
Similar Products
Product Notes
The HMGN1 hmgn1 (Catalog #AAA203453) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's HMGN1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HMGN1 hmgn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD LEHHHHHH. It is sometimes possible for the material contained within the vial of "HMGN1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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