3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase Recombinant Protein | HMGCL recombinant protein
Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase, Sf9
Gene Names
HMGCL; HL
Purity
Greater than 90.0% as determined by SDS-PAGE
Synonyms
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase; Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase; Sf9; 3-Hydroxymethyl-3-Methylglutaryl-Coenzyme A Lyase; 3-Hydroxy-3-Methylglutarate-CoA Lyase; Hydroxymethylglutaricaciduria; HMG-CoA Lyase; EC 4.1.3.4; HL; Mitochondrial 3-Hydroxy-3-Methylglutaryl-CoA Lyase; Hydroxymethylglutaryl-CoA Lyase; mitochondrial; 3-Hydroxy-3-Methylglutaryl-CoA Lyase; Hydroxymethylglutaryl-CoA lyase; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; HMGCL Human; 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase Human Recombinant; HMGCL recombinant protein
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE
Form/Format
Sterile filtered colorless solution
Protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4), 20% glycerol and 1mM DTT
Protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4), 20% glycerol and 1mM DTT
Sequence
M TLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL QKLLEAGNFI CQALNRKTSS KVAQATCKLH HHHHH.
Sequence Length
325
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
Related Product Information for HMGCL recombinant protein
Description: HMGCL Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (28-325 a.a.) and having a molecular mass of 32.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). HMGCL is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: Hydroxymethylglutaryl-CoA lyase (HMGCL) is a mitochondrial matrix protein which is a member of the HMG-CoA lyase family. HMGCL is a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies which, during fasting, provides a major source of energy for the heart, brain and kidney. More precisely, HMGCL catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
Introduction: Hydroxymethylglutaryl-CoA lyase (HMGCL) is a mitochondrial matrix protein which is a member of the HMG-CoA lyase family. HMGCL is a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies which, during fasting, provides a major source of energy for the heart, brain and kidney. More precisely, HMGCL catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
Product Categories/Family for HMGCL recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,222 Da
NCBI Official Full Name
Hydroxymethylglutaryl-CoA lyase, mitochondrial
NCBI Official Synonym Full Names
3-hydroxymethyl-3-methylglutaryl-CoA lyase
NCBI Official Symbol
HMGCL
NCBI Official Synonym Symbols
HL
NCBI Protein Information
hydroxymethylglutaryl-CoA lyase, mitochondrial
UniProt Protein Name
Hydroxymethylglutaryl-CoA lyase, mitochondrial
Protein Family
UniProt Gene Name
HMGCL
UniProt Synonym Gene Names
HL; HMG-CoA lyase
NCBI Description
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Research Articles on HMGCL
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Product Notes
The HMGCL hmgcl (Catalog #AAA147123) is a Recombinant Protein produced from Sf9, Baculovirus cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: M TLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL QKLLEAGNFI CQALNRKTSS KVAQATCKLH HHHHH. It is sometimes possible for the material contained within the vial of "3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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