High Mobility Group Protein B2/HMGB2 Recombinant Protein | HMGB2 recombinant protein

Recombinant Human High Mobility Group Protein B2/HMGB2 Protein (C-6His)

Cat. Num. AAA2552770

• Gene Names: HMGB2; HMG2
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: High Mobility Group Protein B2/HMGB2; Recombinant Human High Mobility Group Protein B2/HMGB2 Protein (C-6His); High Mobility Group Protein B2; High Mobility Group Protein 2; HMG-2; HMGB2; HMG2; HMGB2 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence Positions: Gly2-Glu209
• Sequence Length: 209

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for HMGB2 recombinant protein

High Mobility Group Protein B2 (HMGB2) belongs to the non-histone chromosomal high-mobility group protein family. Members of this family are chromatin-associated and widely spread in the nucleus of higher eukaryotic cells. HMGB2 contains 2 HMG box DNA-binding domains. It is associated with chromatin and has the ability to bend DNA, preferentially single-stranded DNA. It is shown that HMGB2 is able to efficiently bend DNA and form DNA circles. In addition, HMGB2 is involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

NCBI and Uniprot Product Information

• NCBI GI #: 194688133
• NCBI GeneID: 3148
• NCBI Accession #: NP_001124160.1
• NCBI GenBank Nucleotide #: NM_001130688.1
• UniProt Accession #: P26583; Q5U072; B2R4K8; D3DP37
• Molecular Weight: Molecular Mass: 25.1 kDa; Actual Protein Molecular Mass: 28 kDa
• NCBI Official Full Name: high mobility group protein B2
• NCBI Official Synonym Full Names: high mobility group box 2
• NCBI Official Symbol: HMGB2
• NCBI Official Synonym Symbols: HMG2
• NCBI Protein Information: high mobility group protein B2
• UniProt Protein Name: High mobility group protein B2
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB2
• UniProt Synonym Gene Names: HMG2; HMG-2

NCBI Description

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:7797075, PubMed:11909973, PubMed:19522541, PubMed:18413230, PubMed:19965638, PubMed:20123072). Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285). Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed:23877675). Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway ().