Human Lysosomal-associated membrane protein 2 ELISA Kit | HLAMP-2 elisa kit

Human Human Lysosomal-associated membrane protein 2 (HLAMP-2) ELISA Kit

Cat. Num. AAA282060

• Gene Names: LAMP2; LAMPB; CD107b; LAMP-2; LGP-96; LGP110
• Reactivity: Human
• Synonyms: Lysosomal-associated membrane protein 2; Human Human Lysosomal-associated membrane protein 2 (HLAMP-2) ELISA Kit; HLAMP-2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human LAMP2. No significant cross-reactivity or interference between Human LAMP2 and analogues was observed.
• Sequence Length: 407

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for HLAMP-2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LAMP2 in samples. An antibody specific for LAMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 169790833
• NCBI GeneID: 3920
• NCBI Accession #: NP_001116078.1
• NCBI GenBank Nucleotide #: NM_001122606.1
• UniProt Accession #: P13473; Q16641; Q6Q3G8; Q96J30; Q99534; Q9UD93; A8K4X5; D3DTF0
• Molecular Weight: 45,170 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform C
• NCBI Official Synonym Full Names: lysosomal associated membrane protein 2
• NCBI Official Symbol: LAMP2
• NCBI Official Synonym Symbols: LAMPB; CD107b; LAMP-2; LGP-96; LGP110
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• UniProt Gene Name: LAMP2
• UniProt Synonym Gene Names: LAMP-2; Lysosome-associated membrane protein 2

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: extracellular space; late endosome; late endosome membrane; lysosomal lumen; lysosomal membrane; lysosome; membrane; phagocytic vesicle membrane; plasma membrane; platelet dense granule membrane

Molecular Function: enzyme binding; protein binding; protein domain specific binding

Biological Process: cellular response to starvation; muscle maintenance; neutrophil degranulation; platelet degranulation; protein import; protein stabilization; protein targeting; regulation of protein stability

Disease: Danon Disease