Rabbit anti-Human, Rat HLA-DOB Polyclonal Antibody | anti-HLA-DOB antibody

HLA-DOB Polyclonal Antibody

Cat. Num. AAA9132064

• Gene Names: HLA-DOB; DOB; HLA_DOB
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: HLA-DOB; Polyclonal Antibody; HLA-DOB Polyclonal Antibody; DOB; anti-HLA-DOB antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 273

• Applicable Applications for anti-HLA-DOB antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:1000
• Immunogen: Recombinant protein of human HLA-DOB
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HLA-DOB antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Related Product Information for anti-HLA-DOB antibody

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Product Categories/Family for anti-HLA-DOB antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 122261
• NCBI GeneID: 3112
• UniProt Accession #: P13765; Q29746; Q29825; Q6FHC2; B0V0Y0
• Molecular Weight: 31kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO beta
• NCBI Official Symbol: HLA-DOB
• NCBI Official Synonym Symbols: DOB; HLA_DOB
• NCBI Protein Information: HLA class II histocompatibility antigen, DO beta chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DO beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DOB

NCBI Description

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.32

Cellular Component: lysosomal membrane; lysosome; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of antigen processing and presentation of peptide antigen via MHC class II