HIV-1 gp120 recombinant protein
Human HIV-1 gp120 Recombinant
Purity
>90%, as determined by SDS-PAGE.
Synonyms
HIV-1 gp120; Human HIV-1 gp120 Recombinant; hiv1; gp120; HIV-1 gp120 recombinant protein
Host
E Coli
Specificity
immunoreative with sera of infected HIV individuals.
Purity/Purification
>90%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 20mM PBS pH-7.8, 20mM NaCl 0.5M, 1mM DTT & 8M urea.
Concentration
7.5mg/ml (varies by lot)
Sequence Length
138
Application Notes
HIV1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HIV with minimal specificity problems.
Host Note
DNA sequence encoding immunodominant fragment of HIV-1 gp120 was expressed in Escherichia Coli.
Presentation:
0.2um filtered 50mM Tris pH 8.0 NaCl 60mM, 10mM Glutahione, 0.25% Sarkosyl, 50% Glycerol.
Endotoxin:
Less than 01EU/ug as determined by LAL method.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant HIV-1 gp120 encodes the immunodominant sequences of the HIV surface Glycoprotein 120.
Recombinant HIV-1 Glycoprotein 120 contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Recombinant HIV-1 Glycoprotein 120 contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
Stable for at least 1 year from date of receipt at -18 deg. C
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for HIV-1 gp120 recombinant protein
Recombinant HIV-1 Glycoprotein gp120 contains a GST purification tag and migrates as an approximately 35 kDa protein under reducing condition in SDS-PAGE.
Product Categories/Family for HIV-1 gp120 recombinant protein
References
• structure-function analysis of HIV-1 gp120 amino acid mutations associated with resistance to the ccr5 co-receptor antagonist vicriviroc .J. Virol., Sep 2009; 10.1128/JVI.01351-09.
• HIV-1 gp120-induced migration of dendritic cells is regulated by a novel kinase cascade involving pyk2, p38 map kinase and lsp1 .Blood, Aug 2009; 10.1182/blood-2009-02-206342.
• x4 HIV-1 gp120 down-modulates expression and immunogenicity of co-delivered antigens .J. Virol., Aug 2009; 10.1128/JVI.00394-09.
• llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (hiv-1) -neutralizing properties and high affinity for HIV-1 gp120 .J. Virol., Dec 2008; 82: 12069 - 12081.
• cannabinoids inhibit HIV-1 gp120-mediated insults in brain microvascular endothelial cells .J. Immunol., Nov 2008; 181: 6406 - 6416.
• HIV-1 gp120-induced migration of dendritic cells is regulated by a novel kinase cascade involving pyk2, p38 map kinase and lsp1 .Blood, Aug 2009; 10.1182/blood-2009-02-206342.
• x4 HIV-1 gp120 down-modulates expression and immunogenicity of co-delivered antigens .J. Virol., Aug 2009; 10.1128/JVI.00394-09.
• llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (hiv-1) -neutralizing properties and high affinity for HIV-1 gp120 .J. Virol., Dec 2008; 82: 12069 - 12081.
• cannabinoids inhibit HIV-1 gp120-mediated insults in brain microvascular endothelial cells .J. Immunol., Nov 2008; 181: 6406 - 6416.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HIV-1 gp120 (Catalog #AAA553191) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. HIV1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HIV with minimal specificity problems. . Researchers should empirically determine the suitability of the HIV-1 gp120 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gp120, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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