SDS-Page
(Recombinant Histone H3.1 (Human) protein gel. Recombinant Histone H3.1 run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 15.4 kDa Purity: > 90%)
Histone H3.1 Recombinant Protein | H3.1 recombinant protein
Recombinant Histone H3.1 (Human)
Synonyms
Histone H3.1; Recombinant Histone H3.1 (Human); H3.1 recombinant protein
Host
E Coli
Form/Format
Recombinant Histone H3.1 (Human) is supplied in 25mM Tris-HCl pH7.5, 150mM NaCl, and 10% glycerol.
Protein Species
Human
Notes
Recombinant Histone H3.1 (Human) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant Histone H3.1 (Human) protein gel. Recombinant Histone H3.1 run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 15.4 kDa Purity: > 90%)
SDS-Page
(Recombinant Histone H3.1 (Human) protein gel. Recombinant Histone H3.1 run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 15.4 kDa Purity: > 90%)
Related Product Information for H3.1 recombinant protein
Short Description: Recombinant Histone H3.1 (Human) corresponds to the native histone sequence of mammalian histone H3 variant H3.1 and does not contain any amino acid substitutions or residue analogs. Histone H3.1 is known to be important for gene regulation. Recombinant Histone H3.1, Human consists of the full-length sequence of human Histone H3.1 variant (accession number NM_003529). Recombinant Histone H3.1, Human was generated in E Coli and has an observed molecular weight of 15.4 kDa. Recombinant Histone H3.1 can be used to study enzyme kinetics and screen inhibitors.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.
Product Categories/Family for H3.1 recombinant protein
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Product Notes
The H3.1 (Catalog #AAA388076) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Histone H3.1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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