Mononucleosomes H3K36me3 Recombinant Protein | H3K36me3 recombinant protein

Recombinant Mononucleosomes H3K36me3 (MLA)

Cat. Num. AAA389173

• Gene Names: HIST1H3A; H3/A; H3FA
• Synonyms: Mononucleosomes H3K36me3; Recombinant Mononucleosomes H3K36me3 (MLA); H3K36me3 recombinant protein

• Host: E Coli
• Form/Format: Mononucleosomes H3K36me3 (MLA)(20ug protein + 20ug DNA) are supplied in 10mM Tris-HCl pH8.0, 1mM EDTA, 2mM DTT, 20% glycerol.

• Protein Species: Human
• Conjugation: Methylated
• Notes: Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatinin vitro.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant Mononucleosomes H3K36me3 (MLA) SDS-PAGE gel 13% SDS-PAGE Coomassie staining MW: 108 kDa Purity: >95%)

Testing Data

(Recombinant Mononucleosomes H3K36me3 (MLA) agarose gel Recombinant Mononucleosomes H3K36me3 (MLA) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K36me3. Intact mononucleosomes H3K36me3 migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrap histone octamers to form nucleosomes.)

Western Blot (WB)

(Western Blot analysis for Recombinant Mono-nucleosomes H3K36me3 (MLA) Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K36me3 (MLA) (Lane 2) were detected with H3K36me3 antibody and H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K36me3 (MLA) can be detected by H3K36me3 antibody.)

Related Product Information for H3K36me3 recombinant protein

Short Description: Mononucleosomes H3K36me3 (MLA) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NM_003512), H2B that includes amino acids 1-126 (end)(accession number NM_003518), H3 trimethyl Lys36 (H3K36me3)(MLA) that includes amino acids 1-136 (end)(accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end)(accession number NM_003548). All of these histones were expressed in E Coli. The molecular weight of histone octamer is 108 kDa.

Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ?-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

Product Categories/Family for H3K36me3 recombinant protein

Recombinant Proteins; Histone

NCBI and Uniprot Product Information

• NCBI GI #: 4504281
• NCBI GeneID: 8350
• NCBI Accession #: NP_003520.1
• NCBI GenBank Nucleotide #: NM_003512
• UniProt Accession #: P68431; P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3.1
• NCBI Official Synonym Full Names: histone cluster 1, H3a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
• UniProt Protein Name: Histone H3.1
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA
• UniProt Entry Name: H31_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine