His-Tag, Western Blot Positive Control Recombinant Protein
His-Tag, Recombinant, Western Blot Positive Control (6-Histidine)
Applications
Western Blot
Purity
Purified (>95%)
Synonyms
His-Tag; Western Blot Positive Control; Recombinant; Western Blot Positive Control (6-Histidine); Western Blot Positive Control recombinant protein
Host
E. coli
Purity/Purification
Purified (>95%)
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for His-Tag, Western Blot Positive Control recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot. Not suitable for use in ELISA
Other applications not tested.
Western Blot: 10ul/lane. The protein migrates as ~9-10kD band. It will be necessary to have proper size gel (e.g., 10% or 4-10% for proper resolution of small protein. SDS may crystallize in cold conditions. It should redissolve by warming and light mixing at room temperature. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the MBS636427 solution prior to heating and loading on gels. This preparation is not biologically active. Optimal dilutions to be determined by the researcher.
Other applications not tested.
Western Blot: 10ul/lane. The protein migrates as ~9-10kD band. It will be necessary to have proper size gel (e.g., 10% or 4-10% for proper resolution of small protein. SDS may crystallize in cold conditions. It should redissolve by warming and light mixing at room temperature. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the MBS636427 solution prior to heating and loading on gels. This preparation is not biologically active. Optimal dilutions to be determined by the researcher.
Host Note
His-tag-fusion protein, expressed as N-terminal fusion protein of ~10kD in E. coli.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for His-Tag, Western Blot Positive Control recombinant protein
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions (1-4). This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins (1-4). Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
Product Categories/Family for His-Tag, Western Blot Positive Control recombinant protein
Similar Products
Product Notes
The His-Tag, Western Blot Positive Control (Catalog #AAA636427) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His-Tag, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Not suitable for use in ELISA Other applications not tested. Western Blot: 10ul/lane. The protein migrates as ~9-10kD band. It will be necessary to have proper size gel (e.g., 10% or 4-10% for proper resolution of small protein. SDS may crystallize in cold conditions. It should redissolve by warming and light mixing at room temperature. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the MBS636427 solution prior to heating and loading on gels. This preparation is not biologically active. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the His-Tag, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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