Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
HIPK3 recombinant protein
HIPK3, Active
Applications
Western Blot
Synonyms
HIPK3; Active; PKY; YAK1; DYRK6; FIST3; HIPK3 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
Applicable Applications for HIPK3 recombinant protein
Kinase Assay, Western Blot (WB)
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for HIPK3 recombinant protein
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NM_005734.
HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis (1). JNK regulates the expression of HIPK3 in prostate cancer cells and this contributes to increased resistance to Fas receptor-mediated apoptosis by reducing the interaction between FADD and caspase-8 (2). HIPK3 has been reported to phosphorylate FADD and is implicated in multidrug resistance in a number of tumors. HIPK3 increases transcription factor SF-1 activity leading to increased steroidogenic gene expression in response to cAMP signaling.
HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis (1). JNK regulates the expression of HIPK3 in prostate cancer cells and this contributes to increased resistance to Fas receptor-mediated apoptosis by reducing the interaction between FADD and caspase-8 (2). HIPK3 has been reported to phosphorylate FADD and is implicated in multidrug resistance in a number of tumors. HIPK3 increases transcription factor SF-1 activity leading to increased steroidogenic gene expression in response to cAMP signaling.
Product Categories/Family for HIPK3 recombinant protein
References
1. Begley, D.A. et al:Identification and sequence of human PKY, a putative kinase with increased expression in multidrug-resistant cells, with homology to yeast protein kinase Yak1. Gene, 1997; 200 (1-2): 35-43.
2. Curtin, J F. et al: JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells. J Biol Chem. 2004 Apr 23;279(17):17090-100.
2. Curtin, J F. et al: JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells. J Biol Chem. 2004 Apr 23;279(17):17090-100.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~49kDa
Protein Family
Similar Products
Product Notes
The HIPK3 (Catalog #AAA517043) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIPK3 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the HIPK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIPK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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