Rat hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ELISA Kit | HIF1A elisa kit

Rat hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

Cat. Num. AAA704416

• Gene Names: Hif1a; MOP1
• Reactivity: Rat
• Synonyms: hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor); Rat hypoxia-inducible factor 1alpha; HIF-1alpha ELISA Kit; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; alpha; HIF1A elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat HIF-1alpha. No significant cross-reactivity or interference between rat HIF-1alpha and analogues was observed.
• Sequence Length: 823

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: < 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF1A elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 13242249
• NCBI GeneID: 29560
• NCBI Accession #: NP_077335.1
• NCBI GenBank Nucleotide #: NM_024359.1
• UniProt Accession #: O35800; Q9WTU9
• Molecular Weight: 92,319 Da
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha
• NCBI Official Synonym Full Names: hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• NCBI Official Symbol: Hif1a
• NCBI Official Synonym Symbols: MOP1
• NCBI Protein Information: hypoxia-inducible factor 1-alpha; HIF1 alpha; HIF1-alpha; HIF-1 alpha; HIF-1-alpha; hypoxia-inducible factor 1 alpha; hypoxia inducible factor 1, alpha subunit
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif1a
• UniProt Synonym Gene Names: HIF-1-alpha; HIF1-alpha
• UniProt Entry Name: HIF1A_RAT

NCBI Description

regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006]

Uniprot Description

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia