Rat Aryl-Hydrocarbon Receptor Nuclear Translocator 3 ELISA Kit | HIF-3beta/ARNTL elisa kit

Rat Aryl-Hydrocarbon Receptor Nuclear Translocator 3 ELISA Kit

Cat. Num. AAA47377

• Gene Names: ARNT; AHA-1
• Reactivity: Rat
• Synonyms: Aryl-Hydrocarbon Receptor Nuclear Translocator 3; Rat Aryl-Hydrocarbon Receptor Nuclear Translocator 3 ELISA Kit; HIF-3beta/ARNTL elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat HIF-3?/ARNTL and analogues was observed.
• Sequence Length: 790

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25-200pg/ml.
• Sensitivity: 1.0pg/ml.
• Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate HIF-3?/ARNTL concentrations in Rat serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF-3beta/ARNTL elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate HIF-3?/ARNTL concentrations in Rat serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!

NCBI and Uniprot Product Information

• NCBI GI #: 126722727
• NCBI GeneID: 100008996
• NCBI Accession #: NP_001075675.1
• NCBI GenBank Nucleotide #: NM_001082206.1
• UniProt Accession #: O02748
• Molecular Weight: 86,905 Da
• NCBI Official Full Name: aryl hydrocarbon receptor nuclear translocator
• NCBI Official Symbol: ARNT
• NCBI Official Synonym Symbols: AHA-1
• NCBI Protein Information: aryl hydrocarbon receptor nuclear translocator; HIF1-beta; HIF-1 beta; HIF-1-beta; ARNT protein; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1-beta; dioxin receptor, nuclear translocator
• UniProt Protein Name: Aryl hydrocarbon receptor nuclear translocator
• UniProt Gene Name: ARNT
• UniProt Synonym Gene Names: ARNT protein; HIF-1-beta; HIF1-beta
• UniProt Entry Name: ARNT_RABIT

Uniprot Description

Function: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

By similarity.

Subunit structure: Heterodimer with HIF1A

By similarity. Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with NOCA7 and TACC3

By similarity.

Subcellular location: Nucleus.

Tissue specificity: Was expressed at almost the same level in all tissues except for the heart, liver, and small intestine.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.