Hepatitis C Virus NS5, Genotype 2a Recombinant Protein
Hepatitis C Virus NS5, Genotype 2a, Recombinant (HCV)
Purity
Highly Purified95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Hepatitis C Virus NS5; Genotype 2a; Recombinant (HCV); Genotype 2a recombinant protein
Host
E Coli
Specificity
Immunoreactive with sera of HCV-infected individuals
Purity/Purification
Highly Purified
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a liquid in 50mM Tris, pH 8.0, 5mM EDTA.
Application Notes
Recombinant HCV NS5 Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Hepatitis C Virus NS5, Genotype 2a recombinant protein
The hepatitis C virus (HCV) NS5A gene product is a phosphorylated 56- to 58kD nonstructural protein that displays a multitude of activities related to enhancement of viral pathogenesis. Although associated with other viral encoded proteins as part of the viral replicase complex positioned on the cytoplasmic side of the endoplasmic reticulum, a role for NS5A in viral replication has not been defined. Truncated versions of NS5A can act as transcriptional activators, while other recently characterized interactions of NS5A with cellular proteins indicate its pleiotropic role in HCV-host interactions.
It has been shown that NS5B is a membrane-associated protein, which contains a C-terminal domain comprising í21 hydrophobic amino acids that is responsible for membrane anchorage. NS5B may form a complex with cellular proteins or other HCV nonstructural proteins, including NS3, the viral protease and helicase; NS4A, a cofactor of NS3 protease activity; and NS5A, a phosphoprotein containing a putative interferon sensitivity region. Although the HCV replication mechanism is not clearly understood, the essential role of NS5B polymerase in the HCV replication and infection process has been demonstrated in chimpanzees. Accordingly, it has been viewed as an attractive target for antiviral intervention.
The protein contains the HCV NS5 Genotype 1 immunodominant regions, amino acids 2212-2313.
It has been shown that NS5B is a membrane-associated protein, which contains a C-terminal domain comprising í21 hydrophobic amino acids that is responsible for membrane anchorage. NS5B may form a complex with cellular proteins or other HCV nonstructural proteins, including NS3, the viral protease and helicase; NS4A, a cofactor of NS3 protease activity; and NS5A, a phosphoprotein containing a putative interferon sensitivity region. Although the HCV replication mechanism is not clearly understood, the essential role of NS5B polymerase in the HCV replication and infection process has been demonstrated in chimpanzees. Accordingly, it has been viewed as an attractive target for antiviral intervention.
The protein contains the HCV NS5 Genotype 1 immunodominant regions, amino acids 2212-2313.
Product Categories/Family for Hepatitis C Virus NS5, Genotype 2a recombinant protein
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Product Notes
The Hepatitis C Virus NS5, Genotype 2a (Catalog #AAA635640) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Recombinant HCV NS5 Antigen may be used in ELISA and Western Blot, excellent for detection of HCV with minimal specificity problems. Researchers should empirically determine the suitability of the Hepatitis C Virus NS5, Genotype 2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatitis C Virus NS5, Genotype 2a, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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