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SDS-PAGE

HepaCAM2 recombinant protein

Recombinant Rat HepaCAM2 Protein (His tag)

Gene Names
Hepacam2; RGD1305697
Purity
> 95 % as determined by SDS-PAGE
Synonyms
HepaCAM2; Recombinant Rat HepaCAM2 Protein (His tag); HepaCAM2 recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
463
Application Notes
The recombinant rat HepaCAM2 comprises 329 amino acids and predicts a molecular mass of 36.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Leu 33
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for HepaCAM2 recombinant protein
Background: HepaCAM2 belongs to the immunoglobulin family. HepaCAM2, together with HEPACAM1, are cell adhesion molecules. HEPACAM1 modulates cell adhesion and migration. It also inhibits cancer cell growth. HepaCAM2 contains 2 Ig-like C2-type (immunoglobulin-like) domains. The immunoglobulin domain is a type of protein domain that consists of a 2-layer sandwich of between 7 and 9 antiparallel beta-strands arranged in two beta-sheets with a Greek key topology. Members of the immunoglobulin superfamily are found in hundreds of proteins of different functions.

Description: A DNA sequence encoding the rat HepaCAM2 (B5DEN8) (Met1-Leu350) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,211 Da
NCBI Official Full Name
HEPACAM family member 2
NCBI Official Synonym Full Names
HEPACAM family member 2
NCBI Official Symbol
Hepacam2
NCBI Official Synonym Symbols
RGD1305697
NCBI Protein Information
HEPACAM family member 2
UniProt Protein Name
HEPACAM family member 2
Protein Family
UniProt Gene Name
Hepacam2

Uniprot Description

HEPACAM2: Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13

Cellular Component: centrosome; Golgi apparatus; midbody; spindle

Biological Process: centrosome organization and biogenesis

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Product Notes

The HepaCAM2 hepacam2 (Catalog #AAA2547400) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant rat HepaCAM2 comprises 329 amino acids and predicts a molecular mass of 36.7 kDa. The apparent molecular mass of the recombinant protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation. Researchers should empirically determine the suitability of the HepaCAM2 hepacam2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HepaCAM2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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