BCMA Active Protein | TNFRSF17 active protein

BCMA Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8120384

• Gene Names: TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BCMA; BCMA Protein; Human; Recombinant (ECD; His Tag); Human TNFRSF17/BCMA (CD269) Protein (ECD; BCM Protein; CD269 Protein; TNFRSF13A Protein; tumor necrosis factor receptor superfamily member 17; TNFRSF17 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala54

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TNFRSF17 (NP_001183.2)(Met1-Ala54) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Human BAFF at 2ug/mL (100uL/well) can bind Human BCMA. The EC50 of Human BCMA is 30-80ng/mL.; 2. Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF13 at 2ug/mL (100uL/well) can bind Human BCMA. The EC50 of Human BCMA is 0.6-2.1ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Human BAFF at 2 ug/mL (100 ul/well) can bind Human BCMA, The EC50 of Human BCMA is 30-80 ng/mL.)

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF13 at 2 ug/mL (100 ul/well) can bind Human BCMA, The EC50 of Human BCMA is 0.6-2.1 ng/mL.)

Related Product Information for TNFRSF17 active protein

Background: Tumor necrosis factor receptor superfamily, member 17 (TNFRSF17), also known as B cell maturation antigen (BCMA) or CD269 antigen, is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13BBAFF), and to lead to NF-kappaB and MAPK8/JNK activation. TNFRSF17/BCMA/CD269 also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-kappaB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-kappaB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. TNFRSF17/BCMA is a target of donor B-cell immunity in patients with myeloma who respond to DLI. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.

References

Novak AJ, et al. (2004) Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival. Blood. 103 (2): 689-94.O'Connor BP, et al. (2004) BCMA is essential for the survival of long-lived bone marrow plasma cells. J Exp Med. 199(1): 91-8.Moser K, et al. (2006) Stromal niches, plasma cell differentiation and survival. Curr Opin Immunol. 18(3): 265-70.

NCBI and Uniprot Product Information

• NCBI GI #: 23238192
• NCBI GeneID: 608
• NCBI Accession #: NP_001183.2
• NCBI GenBank Nucleotide #: NM_001192.2
• UniProt Accession #: Q02223; Q2TQ40
• Molecular Weight: 14,843 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 17
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 17
• NCBI Official Symbol: TNFRSF17
• NCBI Official Synonym Symbols: BCM; BCMA; CD269; TNFRSF13A
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 17; B cell maturation antigen; B-cell maturation factor; B-cell maturation protein
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 17
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF17
• UniProt Synonym Gene Names: BCM; BCMA
• UniProt Entry Name: TNR17_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF17: Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.

Protein type: Membrane protein, integral; Oncoprotein; Receptor, misc.

Chromosomal Location of Human Ortholog: 16p13.1

Cellular Component: integral to membrane; plasma membrane; endomembrane system

Molecular Function: receptor activity

Biological Process: cell proliferation; immune system process; multicellular organismal development; signal transduction