HAI-1 Cell Lysate | SPINT1 cell lysate
Human HAI-1 HEK293 Overexpression Lysate
Gene Names
SPINT1; HAI; HAI1; MANSC2
Applications
Western Blot
Synonyms
HAI-1; Human HAI-1 HEK293 Overexpression Lysate; Human HAI-1/SPINT1 HEK293 Cell Lysate (WB positive control); serine peptidase inhibitor; Kunitz type 1; Human HAI Overexpression Lysate; Human HAI1 Overexpression Lysate; Human MANSC2 Overexpression Lysate; Human SPINT1 Overexpression Lysate; Human UNQ223/PRO256 Overexpression Lysate; SPINT1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
409aa
Sequence
Met1-Val433
Applicable Applications for SPINT1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SPINT1 isoform 2 (O43278-2) (Met 1-Val 433) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SPINT1 cell lysate
This Human HAI-1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HAI-1 protein from the overexpression lysate was verified.
Product Categories/Family for SPINT1 cell lysate
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,885 Da
NCBI Official Full Name
kunitz-type protease inhibitor 1 isoform 2
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kunitz type 1
NCBI Official Symbol
SPINT1
NCBI Official Synonym Symbols
HAI; HAI1; MANSC2
NCBI Protein Information
kunitz-type protease inhibitor 1
UniProt Protein Name
Kunitz-type protease inhibitor 1
Protein Family
UniProt Gene Name
SPINT1
UniProt Synonym Gene Names
HAI1; HAI-1
UniProt Entry Name
SPIT1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: extracellular region; extracellular space; membrane
Research Articles on SPINT1
Similar Products
Product Notes
The SPINT1 spint1 (Catalog #AAA8114757) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 409aa. AAA Biotech's HAI-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SPINT1 spint1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Val43 3. It is sometimes possible for the material contained within the vial of "HAI-1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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