SLAM/CD150 Cell Lysate | SLAMF1 cell lysate
Rat SLAM/CD150 HEK293 Overexpression Lysate
Gene Names
Slamf1; RGD1560634
Applications
Western Blot
Synonyms
SLAM/CD150; Rat SLAM/CD150 HEK293 Overexpression Lysate; Rat CD150/SLAM/SLAMF1 HEK293 Cell Lysate (WB positive control); signaling lymphocytic activation molecule family member 1; SLAMF1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
456aa
Sequence
Met1-Leu242
Applicable Applications for SLAMF1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Rat
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the rat Slamf1 (NP_001102548.1) (Met1-Leu242) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SLAMF1 cell lysate
CD150/signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phosphoglycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. The CD150 receptor is expressed on thymocytes, activated and memory T cells, B cells, platelets, natural killer T cells, and mature dendritic cells, and is also detected on tumor cells of Hodgkin's lymphoma (HL) and diffuse large B-cell lymphoma with an activated B cell phenotype. Additionally, it is the immune cell receptor for measles virus (MV). As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of interferon gamma (IFN-gamma) in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.
This Rat SLAM/CD150 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAM/CD150 protein from the overexpression lysate was verified.
This Rat SLAM/CD150 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAM/CD150 protein from the overexpression lysate was verified.
Product Categories/Family for SLAMF1 cell lysate
References
Tatsuo H, et al. (2002) The morbillivirus receptor SLAM (CD150). Microbiol Immunol. 46(3): 135-42.Sidorenko SP, et al. (2003)The dual-function CD150 receptor subfamily: the viral attraction. Nat Immunol. 4(1): 19-24.Yurchenko MY, et al. (2010) CD150 regulates JNK1/2 activation in normal and Hodgkin's lymphoma B cells. Immunol Cell Biol. 88(5): 565-74.Leonard VH, et al. (2010) Measles virus selectively blind to signaling lymphocytic activation molecule (SLAM; CD150) is attenuated and induces strong adaptive immune responses in rhesus monkeys. J Virol. 84(7): 3413-20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,409 Da
NCBI Official Full Name
signaling lymphocytic activation molecule
NCBI Official Synonym Full Names
signaling lymphocytic activation molecule family member 1
NCBI Official Symbol
Slamf1
NCBI Official Synonym Symbols
RGD1560634
NCBI Protein Information
signaling lymphocytic activation molecule
UniProt Protein Name
Signaling lymphocytic activation molecule family member 1
Protein Family
UniProt Gene Name
Slamf1
Uniprot Description
SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.3
Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; antigen binding
Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction
Research Articles on SLAMF1
Similar Products
Product Notes
The SLAMF1 slamf1 (Catalog #AAA8117584) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 456aa. AAA Biotech's SLAM/CD150 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SLAMF1 slamf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Leu24 2. It is sometimes possible for the material contained within the vial of "SLAM/CD150, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
