LIMPII/SR-B2 Active Protein | SCARB2 active protein

LIMPII/SR-B2 Protein, Human, Recombinant (His & hFc Tag)

Cat. Num. AAA8120664

• Gene Names: SCARB2; AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII
• Purity: >90% as determined by SDS-PAGE
• Synonyms: LIMPII/SR-B2; LIMPII/SR-B2 Protein; Human; Recombinant (His & hFc Tag); Human LIMP-2/SCARB2/CD36L2 Protein (His & Fc Tag); AMRF Protein; CD36L2 Protein; EPM4 Protein; HLGP85 Protein; LGP85 Protein; LIMP-2 Protein; LIMPII Protein; SR-BII Protein; scavenger receptor class B; member 2; SCARB2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg27-Thr432

• Species: Human
• Activity: Measured by its ability to bind recombinant human RSPO1 in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 27
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the human SCARB2 (NP_005497.1) extracellular domain (Arg 27-Thr 432) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for SCARB2 active protein

Background: Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type I II multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses' propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, and may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.

References

Crombie, R. et al., 1998, J. Biol. Chem. 273: 4855-4863. Febbraio, M. et al., 2001, J. Clin. Invest. 108: 785-791. Kuronita, T. et al., 2002, J. Cell Sci. 115: 4117-4131. Gamp, A.C. et al., 2003, Human Molecular Genetics. 12: 631-646. Eskelinen, E.L. et al., 2003, Trends in Cell Biology. 13: 137-145. Mulcahy, J.V. et al.,2004, Biochem. J. 377 (Pt 3): 741-747. Yamayoshi, S. et al., 2009, Nat Med. 15 (7): 798-801.

NCBI and Uniprot Product Information

• NCBI GI #: 5031631
• NCBI GeneID: 950
• NCBI Accession #: NP_005497.1
• NCBI GenBank Nucleotide #: NM_005506.3
• UniProt Accession #: Q14108; Q53Y63; B4DKD8; E7EM68
• Molecular Weight: 37,767 Da
• NCBI Official Full Name: lysosome membrane protein 2 isoform 1
• NCBI Official Synonym Full Names: scavenger receptor class B, member 2
• NCBI Official Symbol: SCARB2
• NCBI Official Synonym Symbols: AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII
• NCBI Protein Information: lysosome membrane protein 2; LIMP II; CD36 antigen-like 2; lysosome membrane protein II; 85 kDa lysosomal membrane sialoglycoprotein; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; CD36 antigen (collagen type I receptor, thrombos
• UniProt Protein Name: Lysosome membrane protein 2
• Protein Family: Lysosome membrane protein
• UniProt Gene Name: SCARB2
• UniProt Synonym Gene Names: CD36L2; LIMP2; LIMPII; LGP85; LIMP II
• UniProt Entry Name: SCRB2_HUMAN

NCBI Description

The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

SCARB2: Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting. Defects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4). An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved. Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease. Belongs to the CD36 family.

Protein type: Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: lysosomal lumen; focal adhesion; membrane; lysosomal membrane; integral to membrane

Molecular Function: protein binding; enzyme binding; receptor activity

Biological Process: protein targeting to lysosome; cell adhesion

Disease: Epilepsy, Progressive Myoclonic 4, With Or Without Renal Failure