Matrix Metalloproteinase-1 Active Protein | MMP 1 active protein

Recombinant Human Matrix Metalloproteinase-1, HEK

Cat. Num. AAA145376

• Gene Names: MMP1; CLG; CLGN
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Matrix Metalloproteinase-1; Recombinant Human Matrix Metalloproteinase-1; HEK; MMP 1 Human; Matrix Metalloproteinase-1 Human Recombinant; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; MMP1; CLG; CLGN; MMP 1 HEK; MMP 1 active protein

• Host: HEK293 Cells
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: The MMP-1 is supplied as a 0.2 um filtered solution in MES, NaCl, Glycerol and Brij35.; Sterile Filtered colorless solution.
• Sequence Length: 469

• Biological Activity: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/ ug.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50 ug/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37 degree C for 2 hours.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Avoid multiple freeze-thaw cycles.

Related Product Information for MMP 1 active protein

Description: MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-1 is purified by proprietary chromatographic techniques.

Introduction: MMP-1 (interstitial collagenase) can break down a wide range of substrates including types I, II, III, VII, VIII, and X collagens as well as L-Selectin, pro-TNF, IL-1?, IGFBP-3, IGFBP-5, casein, gelatin, ?1 antitrypsin, myelin basic protein, pro-MMP2 and pro-MMP9. A significant function of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling. MMP-1 is expressed in fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 can be divided into a number of distinct domains: a prodomain which is cleaved on activation, a catalytic domain containing the zinc binding site and a short hinge region with a carboxyl terminal domain. MMP1 is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Product Categories/Family for MMP 1 active protein

Enzymes; Matrix Metalloproteinase

NCBI and Uniprot Product Information

• NCBI GI #: 4505215
• NCBI GeneID: 4312
• NCBI Accession #: NP_002412.1
• NCBI GenBank Nucleotide #: NM_002421.3
• UniProt Accession #: P03956; P08156
• Molecular Weight: 54,007 Da
• NCBI Official Full Name: interstitial collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 1
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase; fibroblast collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1
• UniProt Protein Name: Interstitial collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP1
• UniProt Synonym Gene Names: CLG; MMP-1
• UniProt Entry Name: MMP1_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.24.7; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; blood coagulation; proteolysis; leukocyte migration

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive