MMP1 recombinant protein

Recombinant Human MMP1 Protein (His tag)

Cat. Num. AAA2546196

• Gene Names: MMP1; CLG; CLGN
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: MMP1; Recombinant Human MMP1 Protein (His tag); CLG; CLGN; MMP1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM MES, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 5.5
• Sequence Length: 469

• Application Notes: The secreted recombinant human MMP1 comprises 461 amino acids migrates as an approximately 50-55 kDa band as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Phe 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MMP1 recombinant protein

Background: MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of type s I, II, and III at one site in the helical domain. It also cleaves collagens of type s VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

Description: A DNA sequence encoding the pro form of human MMP1 enzyme (NP_002412.1) precursor (Met 1-Asn 469) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4505215
• NCBI GeneID: 4312
• NCBI Accession #: NP_002412.1
• NCBI GenBank Nucleotide #: NM_002421.3
• UniProt Accession #: P03956; P08156
• Molecular Weight: 54,007 Da
• NCBI Official Full Name: interstitial collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 1
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase
• UniProt Protein Name: Interstitial collagenase
• Protein Family: Interstitial collagenase
• UniProt Gene Name: MMP1
• UniProt Synonym Gene Names: CLG; MMP-1

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.7; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: extracellular region

Molecular Function: endopeptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity

Biological Process: cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; leukocyte migration; positive regulation of protein oligomerization; proteolysis

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive