Kallikrein 6 Active Protein | KLK6 active protein

Kallikrein 6 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121097

• Gene Names: KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Kallikrein 6; Kallikrein 6 Protein; Human; Recombinant (His Tag); Human KLK6/Kallikrein 6/Neurosin Protein (His Tag); Bssp Protein; hK6 Protein; Klk7 Protein; PRSS18 Protein; PRSS9 Protein; SP59 Protein; kallikrein-related peptidase 6; KLK6 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys244

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human KLK6 isoform 1 (Q92876-1)(Met 1-Lys 244), was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QARAMC The specific activity is >250 pmols/min/ug. (Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KLK6 active protein

Background: KLK6 (kallikrein-related peptidase 6), also known as Klk7, belongs to the peptidase S1 family, Kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK6 is a serine protease that exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Klk7 shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein, and extracellular matrix proteins such as fibronectin, laminin, vitronectin, and collagen. KLK6 degrades alpha-synuclein and prevents its polymerization, indicating that KLK6 may be involved in the pathogenesis of Parkinson's disease and other synucleinopathies. Klk7 may be involved in the regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.

Product Categories/Family for KLK6 active protein

Serine Proteases and Regulators

References

Krenzer S, et al. (2011) Expression and function of the kallikrein-related peptidase 6 in the human melanoma microenvironment. J Invest Dermatol. 131(11):2281-8.Nathalie HV, et al. (2009) High kallikrein-related peptidase 6 in non-small cell lung cancer cells: an indicator of tumour proliferation and poor prognosis. J Cell Mol Med. 13(9B):4014-22.Kim JT, et al. (2011) Up-regulation and clinical significance of serine protease kallikrein 6 in colon cancer. Cancer. 117(12):2608-19.Scarisbrick IA, et al. (2011) Functional role of kallikrein 6 in regulating immune cell survival. PLoS One. 6(3):e18376.

NCBI and Uniprot Product Information

• NCBI GI #: 61744426
• NCBI GeneID: 5653
• NCBI Accession #: NP_001012983.1
• NCBI GenBank Nucleotide #: NM_001012965.1
• UniProt Accession #: Q92876; Q6H301; A6NJA1; A8MW09
• Molecular Weight: 26,856 Da
• NCBI Official Full Name: kallikrein-6 isoform B
• NCBI Official Synonym Full Names: kallikrein-related peptidase 6
• NCBI Official Symbol: KLK6
• NCBI Official Synonym Symbols: hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• NCBI Protein Information: kallikrein-6; zyme; neurosin; protease M; serine protease 9; serine protease 18
• UniProt Protein Name: Kallikrein-6
• Protein Family: Kallikrein
• UniProt Gene Name: KLK6
• UniProt Synonym Gene Names: PRSS18; PRSS9
• UniProt Entry Name: KLK6_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Nucleolus; Secreted

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; microtubule cytoskeleton; extracellular space; intercellular bridge; nuclear membrane; mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: myelination; collagen catabolic process; protein autoprocessing; central nervous system development; amyloid precursor protein metabolic process; tissue regeneration; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of cell differentiation; response to wounding; hormone metabolic process; protein processing; proteolysis