Integrin alpha X beta 2 Cell Lysate | ITGAX cell lysate

Human Integrin alpha X beta 2 HEK293 Overexpression Lysate

Cat. Num. AAA8118070

• Gene Names: ITGAX; CD11C; SLEB6
• Applications: Western Blot
• Synonyms: Integrin alpha X beta 2; Human Integrin alpha X beta 2 HEK293 Overexpression Lysate; Human ITGAX & ITGB2 Heterodimer CHO Cell Lysate (WB positive control); integrin; alpha X (complement component 3 receptor 4 subunit); Human CD11C Overexpression Lysate; Human SLEB6 Overexpression Lysate; ITGAX cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 1859 (1140+719)aa
• Sequence: Met1-Pro1107, Met1-Asn700

Applicable Applications for ITGAX cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Human

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.

Sequence Construction

A DNA sequence encoding the extracellular domain (Met1-Pro1107) of human ITGAX (P20702) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asn700) of human ITGB2 (P05107), constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.