Granzyme B Cell Lysate | GZMB cell lysate
Human Granzyme B HEK293 Overexpression Lysate
Gene Names
GZMB; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Applications
Western Blot
Synonyms
Granzyme B; Human Granzyme B HEK293 Overexpression Lysate; Human Granzyme B/GZMB HEK293 Cell Lysate (WB positive control); granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1); Human CCPI Overexpression Lysate; Human CGL-1 Overexpression Lysate; Human CGL1 Overexpression Lysate; Human CSP-B Overexpression Lysate; Human CSPB Overexpression Lysate; Human CTLA1 Overexpression Lysate; Human CTSGL1 Overexpression Lysate; Human HLP Overexpression Lysate; Human SECT Overexpression Lysate; GZMB cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
240aa
Sequence
Met1-Tyr247
Applicable Applications for GZMB cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GZMB cell lysate
Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.
This Human Granzyme B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Granzyme B protein from the overexpression lysate was verified.
This Human Granzyme B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Granzyme B protein from the overexpression lysate was verified.
Product Categories/Family for GZMB cell lysate
References
Schmid J., et al., (1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.Trapani J.A., et al., (1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,716 Da
NCBI Official Full Name
granzyme B
NCBI Official Synonym Full Names
granzyme B
NCBI Official Symbol
GZMB
NCBI Official Synonym Symbols
C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
NCBI Protein Information
granzyme B
Protein Family
NCBI Description
This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Research Articles on GZMB
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Product Notes
The GZMB (Catalog #AAA8113744) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 240aa. AAA Biotech's Granzyme B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GZMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Tyr24 7. It is sometimes possible for the material contained within the vial of "Granzyme B, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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